2ygg: Difference between revisions

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[[~~Image~~:~~2ygg~~.~~png~~|~~left~~|~~200px~~]]

==COMPLEX OF CAMBR AND CAM==

== Structural highlights ==

<table><tr><td colspan='2'>[[2ygg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YGG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YGG FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=TAM:TRIS(HYDROXYETHYL)AMINOMETHANE'>TAM</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ygg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ygg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ygg RCSB], [http://www.ebi.ac.uk/pdbsum/2ygg PDBsum]</span></td></tr>

</table>

== Publication Abstract from PubMed ==

The ubiquitous mammalian Na(+)/H(+)-exchanger NHE1 has critical functions in regulating intracellular pH, salt concentration and cellular volume. The regulatory C-terminal domain of NHE1 is linked to the ion-translocating N-terminal membrane domain, and acts as a scaffold for signalling complexes. A major interaction partner is calmodulin (CaM), which binds to two neighbouring regions of NHE1 in a strongly Ca(2+) dependent manner. Upon CaM binding, NHE1 is activated by a shift in sensitivity towards alkaline intracellular pH. Here we report the 2.23 A crystal structure of the NHE1 CaM binding region (NHE1(CaMBR)) in complex with CaM and Ca(2+). The C- and N-lobes of CaM bind the first and second helix of NHE1(CaMBR), respectively. Both the NHE1 helices and Ca(2+)-bound CaM are elongated, as confirmed by small angle X-ray scattering analysis. Our X-ray structure sheds new light on the molecular mechanisms of the phosphorylation-dependent regulation of NHE1 and enables us to propose a model of how Ca(2+) regulates NHE1 activity.

~~{{STRUCTURE_2ygg| PDB=2ygg | SCENE= }}~~

Structure of human Na+/H+ exchanger NHE1 regulatory region in complex with CaM and Ca2+,Koester S, Pavkov-Keller T, Kuehlbrandt W, Yildiz O J Biol Chem. 2011 Sep 19. PMID:21931166<ref>PMID:21931166</ref>

~~===COMPLEX OF CAMBR AND CAM===~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

~~{{ABSTRACT_PUBMED_21931166}}~~

== References ==

~~==About this Structure==~~

__TOC__

~~[[2ygg]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en~~.~~wikipedia~~.~~org~~/~~wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YGG OCA].~~

</StructureSection>

==~~See Also==~~

*[[Calmodulin|Calmodulin]]

~~==Reference~~==

<~~ref group="xtra">PMID:021931166<~~/~~ref~~><~~references group="xtra"~~/>

[[Category: Homo sapiens]]

[[Category: Rattus norvegicus]]

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-[[Image:2ygg.png|left|200px]]
+==COMPLEX OF CAMBR AND CAM==
+<StructureSection load='2ygg' size='340' side='right' caption='[[2ygg]], [[Resolution|resolution]] 2.23&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2ygg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YGG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YGG FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=TAM:TRIS(HYDROXYETHYL)AMINOMETHANE'>TAM</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1y4e|1y4e]], [[2bec|2bec]], [[3cln|3cln]], [[1qx5|1qx5]], [[1qx7|1qx7]], [[1g4y|1g4y]], [[1niw|1niw]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ygg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ygg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ygg RCSB], [http://www.ebi.ac.uk/pdbsum/2ygg PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The ubiquitous mammalian Na(+)/H(+)-exchanger NHE1 has critical functions in regulating intracellular pH, salt concentration and cellular volume. The regulatory C-terminal domain of NHE1 is linked to the ion-translocating N-terminal membrane domain, and acts as a scaffold for signalling complexes. A major interaction partner is calmodulin (CaM), which binds to two neighbouring regions of NHE1 in a strongly Ca(2+) dependent manner. Upon CaM binding, NHE1 is activated by a shift in sensitivity towards alkaline intracellular pH. Here we report the 2.23 A crystal structure of the NHE1 CaM binding region (NHE1(CaMBR)) in complex with CaM and Ca(2+). The C- and N-lobes of CaM bind the first and second helix of NHE1(CaMBR), respectively. Both the NHE1 helices and Ca(2+)-bound CaM are elongated, as confirmed by small angle X-ray scattering analysis. Our X-ray structure sheds new light on the molecular mechanisms of the phosphorylation-dependent regulation of NHE1 and enables us to propose a model of how Ca(2+) regulates NHE1 activity.
-{{STRUCTURE_2ygg|  PDB=2ygg  |  SCENE=  }}
+Structure of human Na+/H+ exchanger NHE1 regulatory region in complex with CaM and Ca2+,Koester S, Pavkov-Keller T, Kuehlbrandt W, Yildiz O J Biol Chem. 2011 Sep 19. PMID:21931166<ref>PMID:21931166</ref>
-===COMPLEX OF CAMBR AND CAM===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_21931166}}
+== References ==
+<references/>
-==About this Structure==
+__TOC__
-[[2ygg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YGG OCA].
+</StructureSection>
-==See Also==
-*[[Calmodulin|Calmodulin]]
-==Reference==
-<ref group="xtra">PMID:021931166</ref><references group="xtra"/>
 [[Category: Homo sapiens]]
 [[Category: Rattus norvegicus]]