1kf2: Difference between revisions
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[[Image:1kf2.jpg|left|200px]] | [[Image:1kf2.jpg|left|200px]] | ||
'''Atomic Resolution Structure of RNase A at pH 5.2''' | {{Structure | ||
|PDB= 1kf2 |SIZE=350|CAPTION= <scene name='initialview01'>1kf2</scene>, resolution 1.10Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] | |||
|GENE= | |||
}} | |||
'''Atomic Resolution Structure of RNase A at pH 5.2''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1KF2 is a [ | 1KF2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KF2 OCA]. | ||
==Reference== | ==Reference== | ||
Atomic resolution structures of ribonuclease A at six pH values., Berisio R, Sica F, Lamzin VS, Wilson KS, Zagari A, Mazzarella L, Acta Crystallogr D Biol Crystallogr. 2002 Mar;58(Pt 3):441-50. Epub 2002, Feb 21. PMID:[http:// | Atomic resolution structures of ribonuclease A at six pH values., Berisio R, Sica F, Lamzin VS, Wilson KS, Zagari A, Mazzarella L, Acta Crystallogr D Biol Crystallogr. 2002 Mar;58(Pt 3):441-50. Epub 2002, Feb 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11856829 11856829] | ||
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Pancreatic ribonuclease]] | [[Category: Pancreatic ribonuclease]] | ||
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[[Category: crystal]] | [[Category: crystal]] | ||
[[Category: ph]] | [[Category: ph]] | ||
[[Category: rnase | [[Category: rnase some]] | ||
[[Category: soaking]] | [[Category: soaking]] | ||
[[Category: titration]] | [[Category: titration]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:16:34 2008'' | ||
Revision as of 10:16, 20 March 2008
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| 1kf2, resolution 1.10Å | |||||||||||||
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| Ligands: | SO4 | ||||||||||||
| Activity: | Pancreatic ribonuclease, with EC number 3.1.27.5 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Atomic Resolution Structure of RNase A at pH 5.2
Overview
The diffraction pattern of protein crystals extending to atomic resolution guarantees a very accurate picture of the molecular structure and enables the study of subtle phenomena related to protein functionality. Six structures of bovine pancreatic ribonuclease at the pH* values 5.2, 5.9, 6.3, 7.1, 8.0 and 8.8 and at resolution limits in the range 1.05-1.15A have been refined. An overall description of the six structures and several aspects, mainly regarding pH-triggered conformational changes, are described here. Since subtle variations were expected, a thorough validation assessment of the six refined models was first carried out. Some stereochemical parameters, such as the N[bond]C(alpha)[bond]C angle and the pyramidalization at the carbonyl C atoms, indicate that the standard target values and their weights typically used in refinement may need revision. A detailed comparison of the six structures has provided experimental evidence on the role of Lys41 in catalysis. Furthermore, insights are given into the structural effects related to the pH-dependent binding of a sulfate anion, which mimics the phosphate group of RNA, in the active site. Finally, the results support a number of thermodynamic and kinetic experimental data concerning the role of the disulfide bridge between Cys65 and Cys72 in the folding of RNase A.
About this Structure
1KF2 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
Atomic resolution structures of ribonuclease A at six pH values., Berisio R, Sica F, Lamzin VS, Wilson KS, Zagari A, Mazzarella L, Acta Crystallogr D Biol Crystallogr. 2002 Mar;58(Pt 3):441-50. Epub 2002, Feb 21. PMID:11856829
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