3nf5: Difference between revisions
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[[Image: | ==Crystal structure of the C-terminal domain of nuclear pore complex component NUP116 from Candida glabrata== | ||
<StructureSection load='3nf5' size='340' side='right' caption='[[3nf5]], [[Resolution|resolution]] 1.94Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3nf5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_glabrata Candida glabrata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NF5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NF5 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | |||
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CAGL0F06171g, NUP116 (gi50287617) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5478 Candida glabrata])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nf5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nf5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3nf5 RCSB], [http://www.ebi.ac.uk/pdbsum/3nf5 PDBsum]</span></td></tr> | |||
</table> | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nf/3nf5_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The nuclear pore complex (NPC), embedded in the nuclear envelope, is a large, dynamic molecular assembly that facilitates exchange of macromolecules between the nucleus and the cytoplasm. The yeast NPC is an eightfold symmetric annular structure composed of ~456 polypeptide chains contributed by ~30 distinct proteins termed nucleoporins. Nup116, identified only in fungi, plays a central role in both protein import and mRNA export through the NPC. Nup116 is a modular protein with N-terminal "FG" repeats containing a Gle2p-binding sequence motif and a NPC targeting domain at its C-terminus. We report the crystal structure of the NPC targeting domain of Candida glabrata Nup116, consisting of residues 882-1034 [CgNup116(882-1034)], at 1.94 A resolution. The X-ray structure of CgNup116(882-1034) is consistent with the molecular envelope determined in solution by small-angle X-ray scattering. Structural similarities of CgNup116(882-1034) with homologous domains from Saccharomyces cerevisiae Nup116, S. cerevisiae Nup145N, and human Nup98 are discussed. | |||
Atomic structure of the nuclear pore complex targeting domain of a Nup116 homologue from the yeast, Candida glabrata.,Sampathkumar P, Kim SJ, Manglicmot D, Bain KT, Gilmore J, Gheyi T, Phillips J, Pieper U, Fernandez-Martinez J, Franke JD, Matsui T, Tsuruta H, Atwell S, Thompson DA, Emtage JS, Wasserman SR, Rout MP, Sali A, Sauder JM, Almo SC, Burley SK Proteins. 2012 Aug;80(8):2110-6. doi: 10.1002/prot.24102. Epub 2012 Jun 4. PMID:22544723<ref>PMID:22544723</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | ==See Also== | ||
*[[Nucleoporin|Nucleoporin]] | *[[Nucleoporin|Nucleoporin]] | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Candida glabrata]] | [[Category: Candida glabrata]] | ||
[[Category: Atwell, S | [[Category: Atwell, S]] | ||
[[Category: Bain, K | [[Category: Bain, K]] | ||
[[Category: Burley, S K | [[Category: Burley, S K]] | ||
[[Category: Emtage, J S | [[Category: Emtage, J S]] | ||
[[Category: Gheyi, T | [[Category: Gheyi, T]] | ||
[[Category: Gilmore, J | [[Category: Gilmore, J]] | ||
[[Category: Manglicmot, D | [[Category: Manglicmot, D]] | ||
[[Category: | [[Category: Structural genomic]] | ||
[[Category: Rout, M | [[Category: Rout, M]] | ||
[[Category: Sali, A | [[Category: Sali, A]] | ||
[[Category: Sampathkumar, P | [[Category: Sampathkumar, P]] | ||
[[Category: Sauder, J M | [[Category: Sauder, J M]] | ||
[[Category: Thompson, D A | [[Category: Thompson, D A]] | ||
[[Category: Wasserman, S | [[Category: Wasserman, S]] | ||
[[Category: Glebs domain]] | [[Category: Glebs domain]] | ||
[[Category: New | [[Category: NYSGXRC, New York SGX Research Center for Structural Genomics]] | ||
[[Category: Nuclear pore complex]] | [[Category: Nuclear pore complex]] | ||
[[Category: Nup116]] | [[Category: Nup116]] | ||
[[Category: | [[Category: PSI, Protein structure initiative]] | ||
[[Category: Protein transport]] | [[Category: Protein transport]] | ||
