3v0e

Crystal structure of Ciona intestinalis voltage sensor-containing phosphatase (Ci-VSP), residues 256-576(C363S)

spinqualitylabelsall models 3v0e, resolution 1.65Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3v0e is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CL, GOL, SO4 Related: 3v0d, 3v0f, 3v0g, 3v0h, 3v0i, 3v0j Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed The Ciona intestinalis voltage-sensing phosphatase (Ci-VSP) couples a voltage-sensing domain (VSD) to a lipid phosphatase that is similar to the tumor suppressor PTEN. How the VSD controls enzyme function has been unclear. Here, we present high-resolution crystal structures of the Ci-VSP enzymatic domain that reveal conformational changes in a crucial loop, termed the 'gating loop', that controls access to the active site by a mechanism in which residue Glu411 directly competes with substrate. Structure-based mutations that restrict gating loop conformation impair catalytic function and demonstrate that Glu411 also contributes to substrate selectivity. Structure-guided mutations further define an interaction between the gating loop and linker that connects the phosphatase to the VSD for voltage control of enzyme activity. Together, the data suggest that functional coupling between the gating loop and the linker forms the heart of the regulatory mechanism that controls voltage-dependent enzyme activation. A glutamate switch controls voltage-sensitive phosphatase function.,Liu L, Kohout SC, Xu Q, Muller S, Kimberlin CR, Isacoff EY, Minor DL Jr Nat Struct Mol Biol. 2012 May 6;19(6):633-41. doi: 10.1038/nsmb.2289. PMID:22562138 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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