3dnj: Difference between revisions
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[[ | ==The structure of the Caulobacter crescentus ClpS protease adaptor protein in complex with a N-end rule peptide== | ||
<StructureSection load='3dnj' size='340' side='right' caption='[[3dnj]], [[Resolution|resolution]] 1.15Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3dnj]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Caulobacter_vibrioides Caulobacter vibrioides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DNJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3DNJ FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">clpS, CC_2467 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=155892 Caulobacter vibrioides])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dnj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dnj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3dnj RCSB], [http://www.ebi.ac.uk/pdbsum/3dnj PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The N-end rule targets specific proteins for destruction in prokaryotes and eukaryotes. Here, we report a crystal structure of a bacterial N-end rule adaptor, ClpS, bound to a peptide mimic of an N-end rule substrate. This structure, which was solved at a resolution of 1.15 A, reveals specific recognition of the peptide alpha-amino group via hydrogen bonding and shows that the peptide's N-terminal tyrosine side chain is buried in a deep hydrophobic cleft that pre-exists on the surface of ClpS. The adaptor side chains that contact the peptide's N-terminal residue are highly conserved in orthologs and in E3 ubiquitin ligases that mediate eukaryotic N-end rule recognition. We show that mutation of critical ClpS contact residues abrogates substrate delivery to and degradation by the AAA+ protease ClpAP, demonstrate that modification of the hydrophobic pocket results in altered N-end rule specificity, and discuss functional implications for the mechanism of substrate delivery. | |||
The molecular basis of N-end rule recognition.,Wang KH, Roman-Hernandez G, Grant RA, Sauer RT, Baker TA Mol Cell. 2008 Nov 7;32(3):406-14. PMID:18995838<ref>PMID:18995838</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
== | __TOC__ | ||
</StructureSection> | |||
[[Category: Caulobacter vibrioides]] | [[Category: Caulobacter vibrioides]] | ||
[[Category: Baker, T A | [[Category: Baker, T A]] | ||
[[Category: Grant, R A | [[Category: Grant, R A]] | ||
[[Category: Roman-Hernandez, G | [[Category: Roman-Hernandez, G]] | ||
[[Category: Sauer, R T | [[Category: Sauer, R T]] | ||
[[Category: Wang, K | [[Category: Wang, K]] | ||
[[Category: Adaptor]] | [[Category: Adaptor]] | ||
[[Category: Peptide binding protein]] | [[Category: Peptide binding protein]] | ||
[[Category: Protein-peptide complex]] | [[Category: Protein-peptide complex]] | ||
Revision as of 07:55, 12 November 2014
The structure of the Caulobacter crescentus ClpS protease adaptor protein in complex with a N-end rule peptide
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