3da0: Difference between revisions
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[[Image: | ==Crystal structure of a cleaved form of a chimeric receptor binding protein from Lactococcal phages subspecies TP901-1 and p2== | ||
<StructureSection load='3da0' size='340' side='right' caption='[[3da0]], [[Resolution|resolution]] 1.65Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3da0]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactococcus_phage_tp901-1 Lactococcus phage tp901-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DA0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3DA0 FirstGlance]. <br> | |||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1zru|1zru]], [[2bsd|2bsd]], [[2bse|2bse]], [[2f0c|2f0c]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3da0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3da0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3da0 RCSB], [http://www.ebi.ac.uk/pdbsum/3da0 PDBsum]</span></td></tr> | |||
</table> | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/da/3da0_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Lactococcus lactis, a gram-positive bacterium widely used by the dairy industry to manufacture cheeses, is subject to infection by a diverse population of virulent phages. We have previously determined the structures of three receptor binding proteins (RBPs) from lactococcal phages TP901-1, p2, and bIL170, each of them having a distinct host range. Virulent phages p2 and bIL170 are classified within the 936 group, while the temperate phage TP901-1 is a member of the genetically distinct P335 polythetic group. These RBPs comprise three domains: the N-terminal domain, binding to the virion particle; a beta-helical linker domain; and the C-terminal domain, bearing the receptor binding site used for host recognition. Here, we have designed, expressed, and determined the structure of an RBP chimera in which the N-terminal and linker RBP domains of phage TP901-1 (P335) are fused to the C-terminal RBP domain of phage p2 (936). This chimera exhibits a stable structure that closely resembles the parental structures, while a slight displacement of the linker made RBP domain adaptation efficient. The receptor binding site is structurally indistinguishable from that of native p2 RBP and binds glycerol with excellent affinity. | |||
Crystal structure of a chimeric receptor binding protein constructed from two lactococcal phages.,Siponen M, Spinelli S, Blangy S, Moineau S, Cambillau C, Campanacci V J Bacteriol. 2009 May;191(10):3220-5. Epub 2009 Mar 13. PMID:19286807<ref>PMID:19286807</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
== | |||
< | |||
[[Category: Lactococcus phage tp901-1]] | [[Category: Lactococcus phage tp901-1]] | ||
[[Category: Blangy, S | [[Category: Blangy, S]] | ||
[[Category: Cambillau, C | [[Category: Cambillau, C]] | ||
[[Category: Campanacci, V | [[Category: Campanacci, V]] | ||
[[Category: Siponen, M I | [[Category: Siponen, M I]] | ||
[[Category: Spinelli, S | [[Category: Spinelli, S]] | ||
[[Category: Vera, L | [[Category: Vera, L]] | ||
[[Category: Lactococcal phage p2]] | [[Category: Lactococcal phage p2]] | ||
[[Category: Lactococcal phage tp901-1 receptor binding protein]] | [[Category: Lactococcal phage tp901-1 receptor binding protein]] | ||
[[Category: Viral protein]] | [[Category: Viral protein]] | ||
Revision as of 08:04, 12 November 2014
Crystal structure of a cleaved form of a chimeric receptor binding protein from Lactococcal phages subspecies TP901-1 and p2
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