2lqa: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[ | ==Solution NMR structure of Asteropsin A from marine sponge Asteropus sp.== | ||
<StructureSection load='2lqa' size='340' side='right' caption='[[2lqa]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2lqa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Asteropus Asteropus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LQA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LQA FirstGlance]. <br> | |||
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3q8j|3q8j]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lqa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lqa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2lqa RCSB], [http://www.ebi.ac.uk/pdbsum/2lqa PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
BACKGROUND: Herein we report the discovery of a cystine-crosslinked peptide from Porifera along with high-quality spatial details accompanied by the description of its unique effect on neuronal calcium influx. METHODS: Asteropsin A (ASPA) was isolated from the marine sponge Asteropus sp., and its structure was independently determined using X-ray crystallography (0.87A) and solution NMR spectroscopy. RESULTS: An N-terminal pyroglutamate modification, uncommon cis proline conformations, and absence of basic residues helped distinguish ASPA from other cystine-crosslinked knot peptides. ASPA enhanced Ca(2+) influx in murine cerebrocortical neuron cells following the addition of the Na(+) channel activator veratridine but did not modify the oscillation frequency or amplitude of neuronal Ca(2+) currents alone. Allosterism at neurotoxin site 2 was not observed, suggesting an alternative to the known Na(+) channel interaction. CONCLUSIONS: Together with a distinct biological activity, the origin of ASPA suggests a new subclass of cystine-rich knot peptides associated with Porifera. GENERAL SIGNIFICANCE: The discovery of ASPA represents a distinctive addition to an emerging subclass of cystine-crosslinked knot peptides from Porifera. | |||
Asteropsin A: An unusual cystine-crosslinked peptide from porifera enhances neuronal Ca(2+) influx.,Li H, Bowling JJ, Fronczek FR, Hong J, Jabba SV, Murray TF, Ha NC, Hamann MT, Jung JH Biochim Biophys Acta. 2012 Nov 29;1830(3):2591-2599. doi:, 10.1016/j.bbagen.2012.11.015. PMID:23201194<ref>PMID:23201194</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
== | __TOC__ | ||
</StructureSection> | |||
[[Category: Asteropus]] | [[Category: Asteropus]] | ||
[[Category: Bowling, J J | [[Category: Bowling, J J]] | ||
[[Category: Hamann, M T | [[Category: Hamann, M T]] | ||
[[Category: Jung, J H | [[Category: Jung, J H]] | ||
[[Category: Li, H | [[Category: Li, H]] | ||
[[Category: Knottin]] | [[Category: Knottin]] | ||
[[Category: Sponge]] | [[Category: Sponge]] | ||
[[Category: Toxin]] | [[Category: Toxin]] | ||
Revision as of 08:17, 12 November 2014
Solution NMR structure of Asteropsin A from marine sponge Asteropus sp.
| ||||||||||||
