2wzf: Difference between revisions

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[[Image:2wzf.png|left|200px]]
==LEGIONELLA PNEUMOPHILA GLUCOSYLTRANSFERASE CRYSTAL STRUCTURE==
<StructureSection load='2wzf' size='340' side='right' caption='[[2wzf]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2wzf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WZF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WZF FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2wzg|2wzg]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wzf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wzf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wzf RCSB], [http://www.ebi.ac.uk/pdbsum/2wzf PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Legionnaires' disease is caused by a lethal colonization of alveolar macrophages with the Gram-negative bacterium Legionella pneumophila. LpGT (L. pneumophila glucosyltransferase; also known as Lgt1) has recently been identified as a virulence factor, shutting down protein synthesis in the human cell by specific glucosylation of EF1A (elongation factor 1A), using an unknown mode of substrate recognition and a retaining mechanism for glycosyl transfer. We have determined the crystal structure of LpGT in complex with substrates, revealing a GT-A fold with two unusual protruding domains. Through structure-guided mutagenesis of LpGT, several residues essential for binding of the UDP-glucose-donor and EF1A-acceptor substrates were identified, which also affected L. pneumophila virulence as demonstrated by microinjection studies. Together, these results suggested that a positively charged EF1A loop binds to a negatively charged conserved groove on the LpGT structure, and that two asparagine residues are essential for catalysis. Furthermore, we showed that two further L. pneumophila glycosyltransferases possessed the conserved UDP-glucose-binding sites and EF1A-binding grooves, and are, like LpGT, translocated into the macrophage through the Icm/Dot (intracellular multiplication/defect in organelle trafficking) system.


{{STRUCTURE_2wzf|  PDB=2wzf  |  SCENE=  }}
Molecular mechanism of elongation factor 1A inhibition by a Legionella pneumophila glycosyltransferase.,Hurtado-Guerrero R, Zusman T, Pathak S, Ibrahim AF, Shepherd S, Prescott A, Segal G, van Aalten DM Biochem J. 2010 Feb 24;426(3):281-92. PMID:20030628<ref>PMID:20030628</ref>


===LEGIONELLA PNEUMOPHILA GLUCOSYLTRANSFERASE CRYSTAL STRUCTURE===
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
{{ABSTRACT_PUBMED_20030628}}
== References ==
 
<references/>
==About this Structure==
__TOC__
[[2wzf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WZF OCA].
</StructureSection>
 
==Reference==
<ref group="xtra">PMID:020030628</ref><references group="xtra"/>
[[Category: Legionella pneumophila]]
[[Category: Legionella pneumophila]]
[[Category: Aalten, D M.F Van.]]
[[Category: Aalten, D M.F Van]]
[[Category: Hurtado-Guerrero, R.]]
[[Category: Hurtado-Guerrero, R]]
[[Category: Ibrahim, A F.M.]]
[[Category: Ibrahim, A F.M]]
[[Category: Pathak, S.]]
[[Category: Pathak, S]]
[[Category: Prescott, A.]]
[[Category: Prescott, A]]
[[Category: Segal, G.]]
[[Category: Segal, G]]
[[Category: Shepherd, S.]]
[[Category: Shepherd, S]]
[[Category: Zusman, T.]]
[[Category: Zusman, T]]
[[Category: Elongation factor 1a]]
[[Category: Elongation factor 1a]]
[[Category: Glucosyltransferase]]
[[Category: Glucosyltransferase]]
[[Category: Transferase]]
[[Category: Transferase]]
[[Category: Virulence factor]]
[[Category: Virulence factor]]
Retrieved from "https://proteopedia.org/2wzf"