1lnl: Difference between revisions
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[[Image:1lnl.gif|left|200px]] | [[Image:1lnl.gif|left|200px]] | ||
'''Structure of deoxygenated hemocyanin from Rapana thomasiana''' | {{Structure | ||
|PDB= 1lnl |SIZE=350|CAPTION= <scene name='initialview01'>1lnl</scene>, resolution 3.30Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene> and <scene name='pdbligand=CU:COPPER (II) ION'>CU</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Structure of deoxygenated hemocyanin from Rapana thomasiana''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1LNL is a [ | 1LNL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rapana_thomasiana Rapana thomasiana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LNL OCA]. | ||
==Reference== | ==Reference== | ||
The structure of a functional unit from the wall of a gastropod hemocyanin offers a possible mechanism for cooperativity., Perbandt M, Guthohrlein EW, Rypniewski W, Idakieva K, Stoeva S, Voelter W, Genov N, Betzel C, Biochemistry. 2003 Jun 3;42(21):6341-6. PMID:[http:// | The structure of a functional unit from the wall of a gastropod hemocyanin offers a possible mechanism for cooperativity., Perbandt M, Guthohrlein EW, Rypniewski W, Idakieva K, Stoeva S, Voelter W, Genov N, Betzel C, Biochemistry. 2003 Jun 3;42(21):6341-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12767214 12767214] | ||
[[Category: Rapana thomasiana]] | [[Category: Rapana thomasiana]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: oxygen]] | [[Category: oxygen]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:32:58 2008'' | ||
Revision as of 10:32, 20 March 2008
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| 1lnl, resolution 3.30Å | |||||||||||||
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| Ligands: | NAG and CU | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Structure of deoxygenated hemocyanin from Rapana thomasiana
Overview
Structure-function relationships in a molluscan hemocyanin have been investigated by determining the crystal structure of the Rapana thomasiana (gastropod) hemocyanin functional unit RtH2e in deoxygenated form at 3.38 A resolution. This is the first X-ray structure of an unit from the wall of the molluscan hemocyanin cylinder. The crystal structure of RtH2e demonstrates molecular self-assembly of six identical molecules forming a regular hexameric cylinder. This suggests how the functional units are ordered in the wall of the native molluscan hemocyanins. The molecular arrangement is stabilized by specific protomer-to-protomer interactions, which are probably typical for the functional units building the wall of the cylinders. A molecular mechanism for cooperative dioxygen binding in molluscan hemocyanins is proposed on the basis of the molecular interactions between the protomers. In particular, the deoxygenated RtH2e structure reveals a tunnel leading from two opposite sides of the molecule to the active site. The tunnel represents a possible entrance pathway for dioxygen molecules. No such tunnels have been observed in the crystal structure of the oxy-Odg, a functional unit from the Octopus dofleini (cephalopod) hemocyanin in oxygenated form.
About this Structure
1LNL is a Single protein structure of sequence from Rapana thomasiana. Full crystallographic information is available from OCA.
Reference
The structure of a functional unit from the wall of a gastropod hemocyanin offers a possible mechanism for cooperativity., Perbandt M, Guthohrlein EW, Rypniewski W, Idakieva K, Stoeva S, Voelter W, Genov N, Betzel C, Biochemistry. 2003 Jun 3;42(21):6341-6. PMID:12767214
Page seeded by OCA on Thu Mar 20 12:32:58 2008
