1lvq: Difference between revisions
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[[Image:1lvq.gif|left|200px]] | [[Image:1lvq.gif|left|200px]] | ||
'''IC3 of CB1 Bound to G(alpha)i''' | {{Structure | ||
|PDB= 1lvq |SIZE=350|CAPTION= <scene name='initialview01'>1lvq</scene> | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
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'''IC3 of CB1 Bound to G(alpha)i''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1LVQ is a [ | 1LVQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LVQ OCA]. | ||
==Reference== | ==Reference== | ||
Cannabinoid receptor-G protein interactions: G(alphai1)-bound structures of IC3 and a mutant with altered G protein specificity., Ulfers AL, McMurry JL, Miller A, Wang L, Kendall DA, Mierke DF, Protein Sci. 2002 Oct;11(10):2526-31. PMID:[http:// | Cannabinoid receptor-G protein interactions: G(alphai1)-bound structures of IC3 and a mutant with altered G protein specificity., Ulfers AL, McMurry JL, Miller A, Wang L, Kendall DA, Mierke DF, Protein Sci. 2002 Oct;11(10):2526-31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12237474 12237474] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Kendall, D A.]] | [[Category: Kendall, D A.]] | ||
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[[Category: cannabinoid 1 receptor (cb1)]] | [[Category: cannabinoid 1 receptor (cb1)]] | ||
[[Category: helix while bound]] | [[Category: helix while bound]] | ||
[[Category: transferred | [[Category: transferred no]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:35:45 2008'' | ||
Revision as of 10:35, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
IC3 of CB1 Bound to G(alpha)i
Overview
The structure of the C-terminal region of the third cytoplasmic loop (IC3) of the cannabinoid receptor one (CB1) bound to G(alphai1) has been determined using transferred nuclear Overhauser effects (NOEs). The wild-type IC3 sequence is helical when associated with G(alphai1). In contrast, a peptide containing the amino-acid inversion, Ala(341)-Leu(342) adopts a single turn. These findings correlate with the attenuated G(i) association of CB1 with the Ala(341)-Leu(342) mutation previously observed in vivo and the diminished stimulation of G(alphai1) GTPase activity by the corresponding peptide demonstrated in vitro here. These results, the first to report the structure of a GPCR domain while associated with G protein, imply the C-terminus of CB1 IC3, a region with high-sequence conservation among G-protein coupled receptors, must be helical for efficient coupling and activation of the G(i) protein.
About this Structure
1LVQ is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
Cannabinoid receptor-G protein interactions: G(alphai1)-bound structures of IC3 and a mutant with altered G protein specificity., Ulfers AL, McMurry JL, Miller A, Wang L, Kendall DA, Mierke DF, Protein Sci. 2002 Oct;11(10):2526-31. PMID:12237474
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