1m5n: Difference between revisions
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[[Image:1m5n.gif|left|200px]] | [[Image:1m5n.gif|left|200px]] | ||
'''Crystal structure of HEAT repeats (1-11) of importin b bound to the non-classical NLS(67-94) of PTHrP''' | {{Structure | ||
|PDB= 1m5n |SIZE=350|CAPTION= <scene name='initialview01'>1m5n</scene>, resolution 2.90Å | |||
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'''Crystal structure of HEAT repeats (1-11) of importin b bound to the non-classical NLS(67-94) of PTHrP''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1M5N is a [ | 1M5N is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M5N OCA]. | ||
==Reference== | ==Reference== | ||
Molecular basis for the recognition of a nonclassical nuclear localization signal by importin beta., Cingolani G, Bednenko J, Gillespie MT, Gerace L, Mol Cell. 2002 Dec;10(6):1345-53. PMID:[http:// | Molecular basis for the recognition of a nonclassical nuclear localization signal by importin beta., Cingolani G, Bednenko J, Gillespie MT, Gerace L, Mol Cell. 2002 Dec;10(6):1345-53. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12504010 12504010] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: Gillespie, M T.]] | [[Category: Gillespie, M T.]] | ||
[[Category: all helical protein]] | [[Category: all helical protein]] | ||
[[Category: heat | [[Category: heat repeat]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:39:13 2008'' | ||
Revision as of 10:39, 20 March 2008
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| 1m5n, resolution 2.90Å | |||||||||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal structure of HEAT repeats (1-11) of importin b bound to the non-classical NLS(67-94) of PTHrP
Overview
Nuclear import of proteins containing a classical nuclear localization signal (NLS) involves NLS recognition by importin alpha, which associates with importin beta via the IBB domain. Other proteins, including parathyroid hormone-related protein (PTHrP), are imported into the nucleus by direct interaction with importin beta. We solved the crystal structure of a fragment of importin beta-1 (1-485) bound to the nonclassical NLS of PTHrP. The structure reveals a second extended cargo binding site on importin beta distinct from the IBB domain binding site. Using a permeabilized cell import assay we demonstrate that importin beta (1-485) can import PTHrP-coupled cargo in a Ran-dependent manner. We propose that this region contains a prototypical nuclear import receptor domain, which could have evolved into the modern importin beta superfamily.
Disease
Known disease associated with this structure: Humoral hypercalcemia of malignancy (1) OMIM:[168470]
About this Structure
1M5N is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Molecular basis for the recognition of a nonclassical nuclear localization signal by importin beta., Cingolani G, Bednenko J, Gillespie MT, Gerace L, Mol Cell. 2002 Dec;10(6):1345-53. PMID:12504010
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