1mi4: Difference between revisions

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[[Image:1mi4.gif|left|200px]]<br /><applet load="1mi4" size="350" color="white" frame="true" align="right" spinBox="true"
[[Image:1mi4.gif|left|200px]]
caption="1mi4, resolution 1.7&Aring;" />
 
'''Glyphosate insensitive G96A mutant EPSP synthase liganded with shikimate-3-phosphate'''<br />
{{Structure
|PDB= 1mi4 |SIZE=350|CAPTION= <scene name='initialview01'>1mi4</scene>, resolution 1.7&Aring;
|SITE=
|LIGAND= <scene name='pdbligand=S3P:SHIKIMATE-3-PHOSPHATE'>S3P</scene> and <scene name='pdbligand=FMT:FORMIC ACID'>FMT</scene>
|ACTIVITY= [http://en.wikipedia.org/wiki/3-phosphoshikimate_1-carboxyvinyltransferase 3-phosphoshikimate 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.19 2.5.1.19]
|GENE= aroA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
}}
 
'''Glyphosate insensitive G96A mutant EPSP synthase liganded with shikimate-3-phosphate'''
 


==Overview==
==Overview==
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==About this Structure==
==About this Structure==
1MI4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=S3P:'>S3P</scene> and <scene name='pdbligand=FMT:'>FMT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/3-phosphoshikimate_1-carboxyvinyltransferase 3-phosphoshikimate 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.19 2.5.1.19] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MI4 OCA].  
1MI4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MI4 OCA].  


==Reference==
==Reference==
How the mutation glycine96 to alanine confers glyphosate insensitivity to 5-enolpyruvyl shikimate-3-phosphate synthase from Escherichia coli., Eschenburg S, Healy ML, Priestman MA, Lushington GH, Schonbrunn E, Planta. 2002 Nov;216(1):129-35. Epub 2002 Nov 12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12430021 12430021]
How the mutation glycine96 to alanine confers glyphosate insensitivity to 5-enolpyruvyl shikimate-3-phosphate synthase from Escherichia coli., Eschenburg S, Healy ML, Priestman MA, Lushington GH, Schonbrunn E, Planta. 2002 Nov;216(1):129-35. Epub 2002 Nov 12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12430021 12430021]
[[Category: 3-phosphoshikimate 1-carboxyvinyltransferase]]
[[Category: 3-phosphoshikimate 1-carboxyvinyltransferase]]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: inside-out alpha-beta barrel]]
[[Category: inside-out alpha-beta barrel]]


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