3j2i: Difference between revisions
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[[ | ==Structure of late pre-60S ribosomal subunits with nuclear export factor Arx1 bound at the peptide exit tunnel== | ||
<StructureSection load='3j2i' size='340' side='right' caption='[[3j2i]], [[Resolution|resolution]] 11.90Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3j2i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J2I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J2I FirstGlance]. <br> | |||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3izf|3izf]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j2i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j2i RCSB], [http://www.ebi.ac.uk/pdbsum/3j2i PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Preribosomal particles evolve in the nucleus through transient interaction with biogenesis factors before export to the cytoplasm. Here, we report the architecture of the late pre-60S particle, purified from Saccharomyces cerevisiae, through Arx1, a nuclear export factor with structural homology to methionine aminopeptidases, or its binding partner Alb1. Cryo-EM reconstruction of the Arx1 particle at 11.9-A resolution reveals regions of extra density on the pre-60S particle attributed to associated biogenesis factors, confirming the immature state of the nascent subunit. One of these densities could be unambiguously assigned to Arx1. Immunoelectron microscopy and UV cross-linking localize Arx1 close to the ribosomal exit tunnel, in direct contact with ES27, a highly dynamic eukaryotic rRNA expansion segment. The binding of Arx1 at the exit tunnel may position this export factor to prevent premature recruitment of ribosome-associated factors active during translation. | |||
Structure of the pre-60S ribosomal subunit with nuclear export factor Arx1 bound at the exit tunnel.,Bradatsch B, Leidig C, Granneman S, Gnadig M, Tollervey D, Bottcher B, Beckmann R, Hurt E Nat Struct Mol Biol. 2012 Nov 11. doi: 10.1038/nsmb.2438. PMID:23142978<ref>PMID:23142978</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
== | __TOC__ | ||
</StructureSection> | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Beckmann, R | [[Category: Beckmann, R]] | ||
[[Category: Boettcher, B | [[Category: Boettcher, B]] | ||
[[Category: Bradatsch, B | [[Category: Bradatsch, B]] | ||
[[Category: Gnaedig, M | [[Category: Gnaedig, M]] | ||
[[Category: Granneman, S | [[Category: Granneman, S]] | ||
[[Category: Hurt, E | [[Category: Hurt, E]] | ||
[[Category: Leidig, C | [[Category: Leidig, C]] | ||
[[Category: Tollervey, D | [[Category: Tollervey, D]] | ||
[[Category: Cryo-em]] | [[Category: Cryo-em]] | ||
[[Category: Pre-ribosome]] | [[Category: Pre-ribosome]] | ||
