3ju0: Difference between revisions
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[[Image: | ==Structure of the arm-type binding domain of HAI7 integrase== | ||
<StructureSection load='3ju0' size='340' side='right' caption='[[3ju0]], [[Resolution|resolution]] 1.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3ju0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pectobacterium_atrosepticum Pectobacterium atrosepticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JU0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JU0 FirstGlance]. <br> | |||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3jtz|3jtz]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">intB, ECA1679 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29471 Pectobacterium atrosepticum])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ju0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ju0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ju0 RCSB], [http://www.ebi.ac.uk/pdbsum/3ju0 PDBsum]</span></td></tr> | |||
</table> | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ju/3ju0_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The structures of the N-terminal domains of two integrases of closely related but not identical asn tDNA-associated genomic islands, Yersinia HPI (high pathogenicity island; encoding siderophore yersiniabactin biosynthesis and transport) and an Erwinia carotovora genomic island with yet unknown function, HAI7, have been resolved. Both integrases utilize a novel four-stranded beta-sheet DNA-binding motif, in contrast to the known proteins that bind their DNA targets by means of three-stranded beta-sheets. Moreover, the beta-sheets in Int(HPI) and Int(HAI7) are longer than those in other integrases, and the structured helical N terminus is positioned perpendicularly to the large C-terminal helix. These differences strongly support the proposal that the integrases of the genomic islands make up a distinct evolutionary branch of the site-specific recombinases that utilize a unique DNA-binding mechanism. | |||
Structures of the arm-type binding domains of HPI and HAI7 integrases.,Szwagierczak A, Antonenka U, Popowicz GM, Sitar T, Holak TA, Rakin A J Biol Chem. 2009 Nov 13;284(46):31664-71. Epub 2009 Sep 8. PMID:19737930<ref>PMID:19737930</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | ==See Also== | ||
*[[Phage integrase|Phage integrase]] | *[[Phage integrase|Phage integrase]] | ||
*[[Resolvase|Resolvase]] | |||
== | == References == | ||
< | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Pectobacterium atrosepticum]] | [[Category: Pectobacterium atrosepticum]] | ||
[[Category: Antonenka, U | [[Category: Antonenka, U]] | ||
[[Category: Holak, T A | [[Category: Holak, T A]] | ||
[[Category: Popowicz, G M | [[Category: Popowicz, G M]] | ||
[[Category: Rakin, A | [[Category: Rakin, A]] | ||
[[Category: Sitar, T | [[Category: Sitar, T]] | ||
[[Category: Szwagierczak, A | [[Category: Szwagierczak, A]] | ||
[[Category: Dna binding protein]] | [[Category: Dna binding protein]] | ||
[[Category: Four stranded beta-sheet]] | [[Category: Four stranded beta-sheet]] | ||
Revision as of 08:41, 8 December 2014
Structure of the arm-type binding domain of HAI7 integrase
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