3i8g: Difference between revisions

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[[Image:3i8g.png|left|200px]]
==Elongation complex of the 70S ribosome with three tRNAs and mRNA. This entry 3I8G contains 30S ribosomal subnit.The 50S ribosomal subunit can be found in PDB entry 3I8F. Molecule B in the same asymmetric unit is deposited as 3I8G (30S) and 3I8F (50S).==
<StructureSection load='3i8g' size='340' side='right' caption='[[3i8g]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3i8g]] is a 25 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I8G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3I8G FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MIA:2-METHYLTHIO-N6-ISOPENTENYL-ADENOSINE-5-MONOPHOSPHATE'>MIA</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3i8f|3i8f]], [[3i8h|3i8h]], [[3i8i|3i8i]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i8g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i8g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3i8g RCSB], [http://www.ebi.ac.uk/pdbsum/3i8g PDBsum]</span></td></tr>
</table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i8/3i8g_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
One key question in protein biosynthesis is how the ribosome couples mRNA and tRNA movements to prevent disruption of weak codon-anticodon interactions and loss of the translational reading frame during translocation. Here we report the complete path of mRNA on the 70S ribosome at the atomic level (3.1-A resolution), and we show that one of the conformational rearrangements that occurs upon transition from initiation to elongation is a narrowing of the downstream mRNA tunnel. This rearrangement triggers formation of a network of interactions between the mRNA downstream of the A-site codon and the elongating ribosome. Our data elucidate the mechanism by which hypermodified nucleoside 2-methylthio-N6 isopentenyl adenosine at position 37 (ms(2)i(6)A37) in tRNA(Phe)(GAA) stabilizes mRNA-tRNA interactions in all three tRNA binding sites. Another network of contacts is formed between this tRNA modification and ribosomal elements surrounding the mRNA E/P kink, resulting in the anchoring of P-site tRNA. These data allow rationalization of how modification deficiencies of ms(2)i(6)A37 in tRNAs may lead to shifts of the translational reading frame.


{{STRUCTURE_3i8g|  PDB=3i8g  |  SCENE=  }}
Structural aspects of messenger RNA reading frame maintenance by the ribosome.,Jenner LB, Demeshkina N, Yusupova G, Yusupov M Nat Struct Mol Biol. 2010 May;17(5):555-60. Epub 2010 Apr 18. PMID:20400952<ref>PMID:20400952</ref>


===Elongation complex of the 70S ribosome with three tRNAs and mRNA. This entry 3I8G contains 30S ribosomal subnit.The 50S ribosomal subunit can be found in PDB entry 3I8F. Molecule B in the same asymmetric unit is deposited as 3I8G (30S) and 3I8F (50S).===
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
{{ABSTRACT_PUBMED_20400952}}
 
==About this Structure==
[[3i8g]] is a 25 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I8G OCA].


==See Also==
==See Also==
*[[Ribosomal protein S10|Ribosomal protein S10]]
*[[Ribosomal protein S11|Ribosomal protein S11]]
*[[Ribosomal protein S12|Ribosomal protein S12]]
*[[Ribosomal protein S13|Ribosomal protein S13]]
*[[Ribosomal protein S14|Ribosomal protein S14]]
*[[Ribosomal protein S15|Ribosomal protein S15]]
*[[Ribosomal protein S16|Ribosomal protein S16]]
*[[Ribosomal protein S17|Ribosomal protein S17]]
*[[Ribosomal protein S18|Ribosomal protein S18]]
*[[Ribosomal protein S19|Ribosomal protein S19]]
*[[Ribosomal protein S2|Ribosomal protein S2]]
*[[Ribosomal protein S20|Ribosomal protein S20]]
*[[Ribosomal protein S3|Ribosomal protein S3]]
*[[Ribosomal protein S4|Ribosomal protein S4]]
*[[Ribosomal protein S5|Ribosomal protein S5]]
*[[Ribosomal protein S6|Ribosomal protein S6]]
*[[Ribosomal protein S7|Ribosomal protein S7]]
*[[Ribosomal protein S8|Ribosomal protein S8]]
*[[Ribosomal protein S9|Ribosomal protein S9]]
*[[Ribosomal protein THX|Ribosomal protein THX]]
*[[Ribosomal protein THX|Ribosomal protein THX]]
 
*[[Ribosome 3D structures|Ribosome 3D structures]]
==Reference==
== References ==
<ref group="xtra">PMID:020400952</ref><ref group="xtra">PMID:020694005</ref><references group="xtra"/>
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
[[Category: Jenner, L B.]]
[[Category: Jenner, L B]]
[[Category: Yusupov, M.]]
[[Category: Yusupov, M]]
[[Category: Yusupova, G.]]
[[Category: Yusupova, G]]
[[Category: 16s ribosomal rna]]
[[Category: 16s ribosomal rna]]
[[Category: Metal-binding]]
[[Category: Metal-binding]]
Retrieved from "https://proteopedia.org/3i8g"