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[[Image:3kks.png|left|200px]]
==Crystal structure of catalytic core domain of BIV integrase in crystal form II==
<StructureSection load='3kks' size='340' side='right' caption='[[3kks]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3kks]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovine_immunodeficiency_virus Bovine immunodeficiency virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KKS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KKS FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3kkr|3kkr]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gag-pol ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11657 Bovine immunodeficiency virus])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kks OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3kks RCSB], [http://www.ebi.ac.uk/pdbsum/3kks PDBsum]</span></td></tr>
</table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kk/3kks_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Integrase plays a critical role in the recombination of viral DNA into the host genome. Therefore, over the past decade, it has been a hot target of drug design in the fight against type 1 human immunodeficiency virus (HIV-1). Bovine immunodeficiency virus (BIV) integrase has the same function as HIV-1 integrase. We have determined crystal structures of the BIV integrase catalytic core domain (CCD) in two different crystal forms at a resolution of 2.45 A and 2.2 A, respectively. In crystal form I, BIV integrase CCD forms a back-to-back dimer, in which the two active sites are on opposite sides. This has also been seen in many of the CCD structures of HIV-1 integrase that were determined previously. However, in crystal form II, BIV integrase CCD forms a novel face-to-face dimer in which the two active sites are close to each other. Strikingly, the distance separating the two active sites is approximately 20 A, a distance that perfectly matches a 5-base pair interval. Based on these data, we propose a model for the interaction of integrase with its target DNA, which is also supported by many published biochemical data. Our results provide important clues for designing new inhibitors against HIV-1.


{{STRUCTURE_3kks|  PDB=3kks  |  SCENE=  }}
Crystal structures of catalytic core domain of BIV integrase: implications for the interaction between integrase and target DNA.,Yao X, Fang S, Qiao W, Geng Y, Shen Y Protein Cell. 2010 Apr;1(4):363-70. Epub 2010 May 8. PMID:21203948<ref>PMID:21203948</ref>


===Crystal structure of catalytic core domain of BIV integrase in crystal form II===
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
{{ABSTRACT_PUBMED_21203948}}
 
==About this Structure==
[[3kks]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovine_immunodeficiency_virus Bovine immunodeficiency virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KKS OCA].


==See Also==
==See Also==
*[[Retroviral Integrase|Retroviral Integrase]]
*[[Retroviral Integrase|Retroviral Integrase]]
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:021203948</ref><references group="xtra"/>
__TOC__
</StructureSection>
[[Category: Bovine immunodeficiency virus]]
[[Category: Bovine immunodeficiency virus]]
[[Category: Shen, Y.]]
[[Category: Shen, Y]]
[[Category: Beta-strands flanked by alpha-helice]]
[[Category: Beta-strands flanked by alpha-helice]]
[[Category: Dna binding protein]]
[[Category: Dna binding protein]]
Retrieved from "https://proteopedia.org/3kks"