1n96: Difference between revisions
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[[Image:1n96.gif|left|200px]] | [[Image:1n96.gif|left|200px]] | ||
'''DIMERIC SOLUTION STRUCTURE OF THE CYCLIC OCTAMER CD(CGCTCATT)''' | {{Structure | ||
|PDB= 1n96 |SIZE=350|CAPTION= <scene name='initialview01'>1n96</scene> | |||
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'''DIMERIC SOLUTION STRUCTURE OF THE CYCLIC OCTAMER CD(CGCTCATT)''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1N96 is a [ | 1N96 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N96 OCA]. | ||
==Reference== | ==Reference== | ||
Four-stranded DNA structure stabilized by a novel G:C:A:T tetrad., Escaja N, Gelpi JL, Orozco M, Rico M, Pedroso E, Gonzalez C, J Am Chem Soc. 2003 May 14;125(19):5654-62. PMID:[http:// | Four-stranded DNA structure stabilized by a novel G:C:A:T tetrad., Escaja N, Gelpi JL, Orozco M, Rico M, Pedroso E, Gonzalez C, J Am Chem Soc. 2003 May 14;125(19):5654-62. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12733903 12733903] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Escaja, N.]] | [[Category: Escaja, N.]] | ||
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[[Category: unusual dna]] | [[Category: unusual dna]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:53:46 2008'' | ||
Revision as of 10:53, 20 March 2008
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DIMERIC SOLUTION STRUCTURE OF THE CYCLIC OCTAMER CD(CGCTCATT)
Overview
The solution structure of a cyclic oligonucleotide d<pCGCTCATT> has been determined by two-dimensional NMR spectroscopy and restrained molecular dynamics. Under the appropriate experimental conditions, this molecule self-associates, forming a symmetric dimer stabilized by four intermolecular Watson-Crick base pairs. The resulting four-stranded structure consists of two G:C:A:T tetrads, formed by facing the minor groove side of the Watson-Crick base-pairs. Most probably, the association of the base-pairs is stabilized by coordinating a Na(+) cation. This is the first time that this novel G:C:A:T tetrad has been found in an oligonucleotide structure. This observation increases considerably the number of sequences that may adopt a four-stranded architecture. Overall, the three-dimensional structure is similar to those observed previously in other quadruplexes formed by minor groove alignment of Watson-Crick base pairs. This resemblance strongly suggests that we may be observing a general motif for DNA-DNA recognition.
About this Structure
1N96 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Four-stranded DNA structure stabilized by a novel G:C:A:T tetrad., Escaja N, Gelpi JL, Orozco M, Rico M, Pedroso E, Gonzalez C, J Am Chem Soc. 2003 May 14;125(19):5654-62. PMID:12733903
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