3t36: Difference between revisions

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[[Image:3t36.png|left|200px]]
==Crystal structure of lytic transglycosylase MltE from Eschericha coli==
<StructureSection load='3t36' size='340' side='right' caption='[[3t36]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3t36]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T36 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T36 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3t1z|3t1z]], [[3t21|3t21]], [[3t4i|3t4i]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">emtA, mltE, sltZ, ycgP, b1193, JW5821 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 Escherichia coli K-12])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t36 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t36 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t36 RCSB], [http://www.ebi.ac.uk/pdbsum/3t36 PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The lytic transglycosylase MltE from Escherichia coli is a periplasmic, outer membrane-attached enzyme that cleaves the beta-1,4-glycosidic bonds between N-acetylmuramic acid and N-acetylglucosamine residues in the cell wall peptidoglycan, producing 1,6-anhydromuropeptides. Here we report three crystal structures of MltE: in a substrate-free state, in a binary complex with chitopentaose, and in a ternary complex with the glycopeptide inhibitor bulgecin A and the murodipeptide N-acetylglucosaminyl-N-acetylmuramyl-l-Ala-d-Glu. The substrate-bound structures allowed a detailed analysis of the saccharide-binding interactions in six subsites of the peptidoglycan-binding groove (subsites -4 to +2) and, combined with site-directed mutagenesis analysis, confirmed the role of Glu64 as catalytic acid/base. The structures permitted the precise modeling of a short glycan strand of eight saccharide residues, providing evidence for two additional subsites (+3 and +4) and revealing the productive conformational state of the substrate at subsites -1 and +1, where the glycosidic bond is cleaved. Full accessibility of the peptidoglycan-binding groove and preferential binding of an N-acetylmuramic acid residue in a (4)C(1) chair conformation at subsite +2 explain why MltE shows only endo- and no exo-specific activity toward glycan strands. The results further indicate that catalysis of glycosidic bond cleavage by MltE proceeds via distortion toward a sofa-like conformation of the N-acetylmuramic acid sugar ring at subsite -1 and by anchimeric assistance of the sugar's N-acetyl group, as shown previously for the lytic transglycosylases Slt70 and MltB.


{{STRUCTURE_3t36|  PDB=3t36  |  SCENE=  }}
On the Mechanism of Peptidoglycan Binding and Cleavage by the endo-Specific Lytic Transglycosylase MltE from Escherichia coli.,Fibriansah G, Gliubich FI, Thunnissen AM Biochemistry. 2012 Oct 30. PMID:23075328<ref>PMID:23075328</ref>


===Crystal structure of lytic transglycosylase MltE from Eschericha coli===
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
{{ABSTRACT_PUBMED_23075328}}
== References ==
 
<references/>
==About this Structure==
__TOC__
[[3t36]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T36 OCA].
</StructureSection>
[[Category: Escherichia coli k-12]]
[[Category: Escherichia coli k-12]]
[[Category: Fibriansah, G.]]
[[Category: Fibriansah, G]]
[[Category: Gliubich, F I.]]
[[Category: Gliubich, F I]]
[[Category: Thunnissen, A-M W.H.]]
[[Category: Thunnissen, A-M W.H]]
[[Category: Goose type lysozyme-like structure]]
[[Category: Goose type lysozyme-like structure]]
[[Category: Lyase]]
[[Category: Lyase]]
[[Category: Lytic transglycosylase]]
[[Category: Lytic transglycosylase]]
Retrieved from "https://proteopedia.org/3t36"