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[[Image:3qhy.png|left|200px]]
==Structural, thermodynamic and kinetic analysis of the picomolar binding affinity interaction of the beta-lactamase inhibitor protein-II (BLIP-II) with class A beta-lactamases==
<StructureSection load='3qhy' size='340' side='right' caption='[[3qhy]], [[Resolution|resolution]] 2.06&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3qhy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis] and [http://en.wikipedia.org/wiki/Streptomyces_exfoliatus Streptomyces exfoliatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QHY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QHY FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1jtd|1jtd]], [[1jtg|1jtg]], [[3qi0|3qi0]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bla1, BA_2507, GBAA2507, GBAA_2507 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 Bacillus anthracis]), bliB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1905 Streptomyces exfoliatus])</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qhy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qhy RCSB], [http://www.ebi.ac.uk/pdbsum/3qhy PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
beta-Lactamases hydrolyze beta-lactam antibiotics to provide drug resistance to bacteria. beta-Lactamase inhibitory protein-II (BLIP-II) is a potent proteinaceous inhibitor that exhibits low picomolar affinity for class A beta-lactamases. This study examines the driving forces for binding between BLIP-II and beta-lactamases using a combination of presteady state kinetics, isothermal titration calorimetry, and x-ray crystallography. The measured dissociation rate constants for BLIP-II and various beta-lactamases ranged from 10(-4) to 10(-7) s(-1) and are comparable with those found in some of the tightest known protein-protein interactions. The crystal structures of BLIP-II alone and in complex with Bacillus anthracis Bla1 beta-lactamase revealed no significant side-chain movement in BLIP-II in the complex versus the monomer. The structural rigidity of BLIP-II minimizes the loss of the entropy upon complex formation and, as indicated by thermodynamics experiments, may be a key determinant of the observed potent inhibition of beta-lactamases.


{{STRUCTURE_3qhy|  PDB=3qhy  |  SCENE=  }}
Analysis of the binding forces driving the tight interactions between beta-lactamase inhibitory protein-II (BLIP-II) and class A beta-lactamases.,Brown NG, Chow DC, Sankaran B, Zwart P, Prasad BV, Palzkill T J Biol Chem. 2011 Sep 16;286(37):32723-35. Epub 2011 Jul 20. PMID:21775426<ref>PMID:21775426</ref>


===Structural, thermodynamic and kinetic analysis of the picomolar binding affinity interaction of the beta-lactamase inhibitor protein-II (BLIP-II) with class A beta-lactamases===
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
{{ABSTRACT_PUBMED_21775426}}
 
==About this Structure==
[[3qhy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis] and [http://en.wikipedia.org/wiki/Streptomyces_exfoliatus Streptomyces exfoliatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QHY OCA].


==See Also==
==See Also==
*[[Beta-lactamase|Beta-lactamase]]
*[[TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)|TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)]]
*[[TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)|TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)]]
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:021775426</ref><references group="xtra"/>
__TOC__
</StructureSection>
[[Category: Bacillus anthracis]]
[[Category: Bacillus anthracis]]
[[Category: Beta-lactamase]]
[[Category: Beta-lactamase]]
[[Category: Streptomyces exfoliatus]]
[[Category: Streptomyces exfoliatus]]
[[Category: BSGC, Berkeley Structural Genomics Center.]]
[[Category: Structural genomic]]
[[Category: Brown, N G.]]
[[Category: Brown, N G]]
[[Category: Chow, D C.]]
[[Category: Chow, D C]]
[[Category: Palzkill, T.]]
[[Category: Palzkill, T]]
[[Category: Prasad, B V.V.]]
[[Category: Prasad, B V.V]]
[[Category: Sankaran, B.]]
[[Category: Sankaran, B]]
[[Category: Zwart, P.]]
[[Category: Zwart, P]]
[[Category: Beta-lactamase]]
[[Category: Beta-propeller]]
[[Category: Beta-propeller]]
[[Category: Enyzme-inhibitor complex]]
[[Category: Enyzme-inhibitor complex]]
[[Category: Hydrolase-hydrolase inhibitor complex]]
[[Category: Hydrolase-hydrolase inhibitor complex]]
[[Category: Protein:protein interaction]]
[[Category: Protein:protein interaction]]
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