1nco: Difference between revisions
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[[Image:1nco.gif|left|200px]] | [[Image:1nco.gif|left|200px]] | ||
'''STRUCTURE OF THE ANTITUMOR PROTEIN-CHROMOPHORE COMPLEX NEOCARZINOSTATIN''' | {{Structure | ||
|PDB= 1nco |SIZE=350|CAPTION= <scene name='initialview01'>1nco</scene>, resolution 1.8Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=CHR:NEOCARZINOSTATIN-CHROMOPHORE'>CHR</scene> and <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''STRUCTURE OF THE ANTITUMOR PROTEIN-CHROMOPHORE COMPLEX NEOCARZINOSTATIN''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1NCO is a [ | 1NCO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_carzinostaticus Streptomyces carzinostaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NCO OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of neocarzinostatin, an antitumor protein-chromophore complex., Kim KH, Kwon BM, Myers AG, Rees DC, Science. 1993 Nov 12;262(5136):1042-6. PMID:[http:// | Crystal structure of neocarzinostatin, an antitumor protein-chromophore complex., Kim KH, Kwon BM, Myers AG, Rees DC, Science. 1993 Nov 12;262(5136):1042-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8235619 8235619] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Streptomyces carzinostaticus]] | [[Category: Streptomyces carzinostaticus]] | ||
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[[Category: antibacterial and antitumor protein]] | [[Category: antibacterial and antitumor protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:55:05 2008'' | ||
Revision as of 10:55, 20 March 2008
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| 1nco, resolution 1.8Å | |||||||||||||
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| Ligands: | CHR and MRD | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
STRUCTURE OF THE ANTITUMOR PROTEIN-CHROMOPHORE COMPLEX NEOCARZINOSTATIN
Overview
Structures of the protein-chromophore complex and the apoprotein form of neocarzinostatin were determined at 1.8 angstrom resolution. Neocarzinostatin is composed of a labile chromophore with DNA-cleaving activity and a stabilizing protein. The chromophore displays marked nonlinearity of the triple bonds and is bound noncovalently in a pocket formed by the two protein domains. The chromophore pi-face interacts with the phenyl ring edges of Phe52 and Phe78. The amino sugar and carbonate groups of the chromophore are solvent exposed, whereas the epoxide, acetylene groups, and carbon C-12, the site of nucleophilic thiol addition during chromophore activation, are unexposed. The position of the amino group of the chromophore carbohydrate relative to C-12 supports the idea that the amino group plays a role in thiol activation.
About this Structure
1NCO is a Single protein structure of sequence from Streptomyces carzinostaticus. Full crystallographic information is available from OCA.
Reference
Crystal structure of neocarzinostatin, an antitumor protein-chromophore complex., Kim KH, Kwon BM, Myers AG, Rees DC, Science. 1993 Nov 12;262(5136):1042-6. PMID:8235619
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