3nzm: Difference between revisions
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[[ | ==Crystal structure of DNAE intein with N-extein in redox trap== | ||
<StructureSection load='3nzm' size='340' side='right' caption='[[3nzm]], [[Resolution|resolution]] 1.55Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3nzm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Synechocystis_sp._pcc_6803 Synechocystis sp. pcc 6803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NZM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NZM FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SNN:L-3-AMINOSUCCINIMIDE'>SNN</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1zde|1zde]], [[1zd7|1zd7]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">dnaE-N, slr0603, dnaE-C, sll1572 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1148 Synechocystis sp. PCC 6803])</td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nzm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nzm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3nzm RCSB], [http://www.ebi.ac.uk/pdbsum/3nzm PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Here we describe self-splicing proteins, called inteins, that function as redox-responsive switches in bacteria. Redox regulation was achieved by engineering a disulfide bond between the intein's catalytic cysteine and a cysteine in the flanking 'extein' sequence. This interaction was validated by an X-ray structure, which includes a transient splice junction. A natural analog of the designed system was identified in Pyrococcus abyssi, suggesting an unprecedented form of adaptive, post-translational regulation. | |||
Structure of catalytically competent intein caught in a redox trap with functional and evolutionary implications.,Callahan BP, Topilina NI, Stanger MJ, Van Roey P, Belfort M Nat Struct Mol Biol. 2011 May;18(5):630-3. Epub 2011 Apr 3. PMID:21460844<ref>PMID:21460844</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | ==See Also== | ||
*[[DNA polymerase|DNA polymerase]] | *[[DNA polymerase|DNA polymerase]] | ||
== References == | |||
== | <references/> | ||
< | __TOC__ | ||
</StructureSection> | |||
[[Category: DNA-directed DNA polymerase]] | [[Category: DNA-directed DNA polymerase]] | ||
[[Category: Synechocystis sp. pcc 6803]] | [[Category: Synechocystis sp. pcc 6803]] | ||
[[Category: Belfort, M | [[Category: Belfort, M]] | ||
[[Category: Callahan, B P | [[Category: Callahan, B P]] | ||
[[Category: Roey, P Van | [[Category: Roey, P Van]] | ||
[[Category: Disulfide bond]] | [[Category: Disulfide bond]] | ||
[[Category: Transferase]] | [[Category: Transferase]] | ||
Revision as of 12:21, 9 December 2014
Crystal structure of DNAE intein with N-extein in redox trap
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