3ous: Difference between revisions
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[[ | ==MthK channel pore T59A mutant== | ||
<StructureSection load='3ous' size='340' side='right' caption='[[3ous]], [[Resolution|resolution]] 1.75Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3ous]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OUS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OUS FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mthK, MTH_1520 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=145262 Methanothermobacter thermautotrophicus])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ous FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ous OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ous RCSB], [http://www.ebi.ac.uk/pdbsum/3ous PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Selective ion conduction across ion channel pores is central to cellular physiology. To understand the underlying principles of ion selectivity in tetrameric cation channels, we engineered a set of cation channel pores based on the nonselective NaK channel and determined their structures to high resolution. These structures showcase an ensemble of selectivity filters with a various number of contiguous ion binding sites ranging from 2 to 4, with each individual site maintaining a geometry and ligand environment virtually identical to that of equivalent sites in K(+) channel selectivity filters. Combined with single channel electrophysiology, we show that only the channel with four ion binding sites is K(+) selective, whereas those with two or three are nonselective and permeate Na(+) and K(+) equally well. These observations strongly suggest that the number of contiguous ion binding sites in a single file is the key determinant of the channel's selectivity properties and the presence of four sites in K(+) channels is essential for highly selective and efficient permeation of K(+) ions. | |||
Tuning the ion selectivity of tetrameric cation channels by changing the number of ion binding sites.,Derebe MG, Sauer DB, Zeng W, Alam A, Shi N, Jiang Y Proc Natl Acad Sci U S A. 2010 Dec 27. PMID:21187421<ref>PMID:21187421</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | ==See Also== | ||
*[[Potassium Channel|Potassium Channel]] | *[[Potassium Channel|Potassium Channel]] | ||
== References == | |||
== | <references/> | ||
< | __TOC__ | ||
</StructureSection> | |||
[[Category: Methanothermobacter thermautotrophicus]] | [[Category: Methanothermobacter thermautotrophicus]] | ||
[[Category: Alam, A | [[Category: Alam, A]] | ||
[[Category: Derebe, M G | [[Category: Derebe, M G]] | ||
[[Category: Jiang, Y | [[Category: Jiang, Y]] | ||
[[Category: Sauer, D B | [[Category: Sauer, D B]] | ||
[[Category: Shi, N | [[Category: Shi, N]] | ||
[[Category: Zeng, W | [[Category: Zeng, W]] | ||
[[Category: Ion channel]] | [[Category: Ion channel]] | ||
[[Category: Membrane]] | [[Category: Membrane]] | ||
[[Category: Membrane protein]] | [[Category: Membrane protein]] | ||
