1nio: Difference between revisions
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[[Image:1nio.gif|left|200px]] | [[Image:1nio.gif|left|200px]] | ||
'''Crystal structure of beta-luffin, a ribosome inactivating protein at 2.0A resolution''' | {{Structure | ||
|PDB= 1nio |SIZE=350|CAPTION= <scene name='initialview01'>1nio</scene>, resolution 2.00Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] | |||
|GENE= | |||
}} | |||
'''Crystal structure of beta-luffin, a ribosome inactivating protein at 2.0A resolution''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1NIO is a [ | 1NIO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Luffa_aegyptiaca Luffa aegyptiaca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NIO OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of beta-luffin, a ribosome-inactivating protein, at 2.0 A resolution., Ma QJ, Li JH, Li HG, Wu S, Dong YC, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1366-70. Epub 2003, Jul 23. PMID:[http:// | Crystal structure of beta-luffin, a ribosome-inactivating protein, at 2.0 A resolution., Ma QJ, Li JH, Li HG, Wu S, Dong YC, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1366-70. Epub 2003, Jul 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12876337 12876337] | ||
[[Category: Luffa aegyptiaca]] | [[Category: Luffa aegyptiaca]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: ribosome inactivating protein(rip)]] | [[Category: ribosome inactivating protein(rip)]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:57:28 2008'' | ||
Revision as of 10:57, 20 March 2008
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| 1nio, resolution 2.00Å | |||||||||||||
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| Ligands: | NAG | ||||||||||||
| Activity: | rRNA N-glycosylase, with EC number 3.2.2.22 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal structure of beta-luffin, a ribosome inactivating protein at 2.0A resolution
Overview
The crystal structure of beta-luffin at 2.0 A resolution was solved by the molecular-replacement method using polyalanyl trichosanthin as the search model. The structure was refined with CNS1.1, giving R(work) = 0.162 and R(free) = 0.204. The r.m.s.d.s of the bond lengths and bond angles are 0.008 A and 1.3 degrees, respectively. The overall structure is similar to those of other type I RIPs. Three N-acetylglucosamine (Nag) molecules are linked to residues Asn2, Asn78 and Asn85 of the protein.
About this Structure
1NIO is a Single protein structure of sequence from Luffa aegyptiaca. Full crystallographic information is available from OCA.
Reference
Crystal structure of beta-luffin, a ribosome-inactivating protein, at 2.0 A resolution., Ma QJ, Li JH, Li HG, Wu S, Dong YC, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1366-70. Epub 2003, Jul 23. PMID:12876337
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