1vyb: Difference between revisions

The human L1 endonuclease (L1-EN) is encoded by the non-LTR, retrotransposon LINE-1 (L1). L1 is responsible for more than 1.5 million, retrotransposition events in the history of the human genome, contributing, more than a quarter to human genomic DNA (L1 and Alu elements). L1-EN is, related to the well-understood human DNA repair endonuclease APE1, and its, nicking specificity is a major determinant for retrotransposon integration, site selection. The crystal structure of human L1 endonuclease is the, first of a retrotransposon-encoded protein and a prototype for, retrotransposon-encoded endonucleases involved in target-primed reverse, transcription. Structure-based endonuclease alignments reveal a conserved, threonine in addition to previously identified invariant residues and, suggest that DNA recognition proceeds via the accommodation of an, extrahelical nucleotide within a pocket of the enzyme. The present, analysis will help to refine phylogenetic and functional relationships, among metal-dependent phosphohydrolases and provides a basis for, manipulating non-LTR retrotransposon integration site selection.

1VYB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4, SO3 and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1VYB OCA].  

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