4f7z: Difference between revisions
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[[ | ==Conformational dynamics of exchange protein directly activated by cAMP== | ||
<StructureSection load='4f7z' size='340' side='right' caption='[[4f7z]], [[Resolution|resolution]] 2.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4f7z]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F7Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F7Z FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Rapgef4, Cgef2, Epac2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f7z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f7z RCSB], [http://www.ebi.ac.uk/pdbsum/4f7z PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Exchange proteins directly activated by cAMP (EPACs) are important allosteric regulators of cAMP-mediated signal transduction pathways. To understand the molecular mechanism of EPAC activation, we have combined site-directed mutagenesis, X-ray crystallography, and peptide amide hydrogen/deuterium exchange mass spectrometry (DXMS) to probe the structural and conformational dynamics of EPAC2-F435G, a constitutively active EPAC2 mutant. Our study demonstrates that conformational dynamics plays a critical role in cAMP-induced EPAC activation. A glycine mutation at 435 position shifts the equilibrium of conformational dynamics towards the extended active conformation. | |||
Structural analyses of a constitutively active mutant of exchange protein directly activated by cAMP.,White MA, Li S, Tsalkova T, Mei FC, Liu T, Woods VL Jr, Cheng X PLoS One. 2012;7(11):e49932. doi: 10.1371/journal.pone.0049932. Epub 2012 Nov 26. PMID:23189173<ref>PMID:23189173</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
== | __TOC__ | ||
</StructureSection> | |||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Cheng, X | [[Category: Cheng, X]] | ||
[[Category: Liu, T | [[Category: Liu, T]] | ||
[[Category: Mei, F C | [[Category: Mei, F C]] | ||
[[Category: Tsalkova, T N | [[Category: Tsalkova, T N]] | ||
[[Category: White, M A | [[Category: White, M A]] | ||
[[Category: Woods, V L | [[Category: Woods, V L]] | ||
[[Category: Auto-inhibition]] | [[Category: Auto-inhibition]] | ||
[[Category: Cdc25 homology domain]] | [[Category: Cdc25 homology domain]] | ||
Revision as of 14:42, 9 December 2014
Conformational dynamics of exchange protein directly activated by cAMP
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