4amc: Difference between revisions
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[[ | ==Crystal structure of Lactobacillus reuteri 121 N-terminally truncated glucansucrase GTFA== | ||
<StructureSection load='4amc' size='340' side='right' caption='[[4amc]], [[Resolution|resolution]] 3.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4amc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactobacillus_reuteri Lactobacillus reuteri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AMC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AMC FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dextransucrase Dextransucrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.5 2.4.1.5] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4amc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4amc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4amc RCSB], [http://www.ebi.ac.uk/pdbsum/4amc PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The reuteransucrase GTFA from Lactobacillus reuteri 121, which belongs to glycosyl hydrolase family GH70, synthesizes branched alpha-glucans with both alpha-1,6- and alpha-1,4-glycosidic linkages (reuteran) from sucrose. The crystal structure of GTFA-DeltaN, a 118 kDa fragment of GTFA comprising residues 745-1763 and including the catalytic domain, was determined at 3.6 A resolution by molecular replacement. The crystals have large solvent channels and an unusually high solvent content of 85%. GTFA-DeltaN has the same domain arrangement and domain topologies as observed in previously determined GH70 glucansucrase structures. The architecture of the GTFA-DeltaN active site and binding pocket confirms that glucansucrases have a conserved substrate specificity for sucrose. However, this first crystal structure of an alpha-1,6/alpha-1,4-specific glucansucrase shows that residues from conserved sequence motif IV (1128-1136 in GTFA-DeltaN) contribute to the acceptor-binding subsites and that they display differences compared with other structurally characterized glucansucrases. In particular, the structure clarifies the importance of residues following the transition-state stabilizer for product specificity, and especially residue Asn1134, which is in a position to interact with sugar units in acceptor subsite +2. | |||
Structure of the alpha-1,6/alpha-1,4-specific glucansucrase GTFA from Lactobacillus reuteri 121.,Pijning T, Vujicic-Zagar A, Kralj S, Dijkhuizen L, Dijkstra BW Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Dec 1;68(Pt 12):1448-54., doi: 10.1107/S1744309112044168. Epub 2012 Nov 14. PMID:23192022<ref>PMID:23192022</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
== | __TOC__ | ||
</StructureSection> | |||
[[Category: Dextransucrase]] | [[Category: Dextransucrase]] | ||
[[Category: Lactobacillus reuteri]] | [[Category: Lactobacillus reuteri]] | ||
[[Category: Dijkhuizen, L | [[Category: Dijkhuizen, L]] | ||
[[Category: Dijkstra, B W | [[Category: Dijkstra, B W]] | ||
[[Category: Kralj, S | [[Category: Kralj, S]] | ||
[[Category: Pijning, T | [[Category: Pijning, T]] | ||
[[Category: Vujicic-Zagar, A | [[Category: Vujicic-Zagar, A]] | ||
[[Category: Glycosyltransferase]] | [[Category: Glycosyltransferase]] | ||
[[Category: Reuteransucrase]] | [[Category: Reuteransucrase]] | ||
[[Category: Transferase]] | [[Category: Transferase]] | ||
