4b86: Difference between revisions
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[[ | ==Crystal structure of the MSL1-MSL2 complex (3.5A)== | ||
<StructureSection load='4b86' size='340' side='right' caption='[[4b86]], [[Resolution|resolution]] 3.50Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4b86]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Buakea_kaeuae Buakea kaeuae] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B86 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B86 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4b7y|4b7y]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b86 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b86 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4b86 RCSB], [http://www.ebi.ac.uk/pdbsum/4b86 PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The Male-Specific Lethal (MSL) complex regulates dosage compensation of the male X chromosome in Drosophila. Here, we report the crystal structure of its MSL1/MSL2 core, where two MSL2 subunits bind to a dimer formed by two molecules of MSL1. Analysis of structure-based mutants revealed that MSL2 can only interact with the MSL1 dimer, but MSL1 dimerization is MSL2 independent. We show that Msl1 is a substrate for Msl2 E3 ubiquitin ligase activity. ChIP experiments revealed that Msl1 dimerization is essential for targeting and spreading of the MSL complex on X-linked genes; however, Msl1 binding to promoters of male and female cells is independent of the dimer status and other MSL proteins. Finally, we show that loss of Msl1 dimerization leads to male-specific lethality. We propose that Msl1-mediated dimerization of the entire MSL complex is required for Msl2 binding, X chromosome recognition, and spreading along the X chromosome. | |||
Msl1-mediated dimerization of the dosage compensation complex is essential for male X-chromosome regulation in Drosophila.,Hallacli E, Lipp M, Georgiev P, Spielman C, Cusack S, Akhtar A, Kadlec J Mol Cell. 2012 Nov 30;48(4):587-600. doi: 10.1016/j.molcel.2012.09.014. Epub 2012, Oct 18. PMID:23084835<ref>PMID:23084835</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
== | __TOC__ | ||
</StructureSection> | |||
[[Category: Buakea kaeuae]] | [[Category: Buakea kaeuae]] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Akhtar, A | [[Category: Akhtar, A]] | ||
[[Category: Cusack, S | [[Category: Cusack, S]] | ||
[[Category: Georgiev, P | [[Category: Georgiev, P]] | ||
[[Category: Hallacli, E | [[Category: Hallacli, E]] | ||
[[Category: Kadlec, J | [[Category: Kadlec, J]] | ||
[[Category: Lipp, M | [[Category: Lipp, M]] | ||
[[Category: Spielman, C | [[Category: Spielman, C]] | ||
[[Category: Chromatin]] | [[Category: Chromatin]] | ||
[[Category: Dosage compensation]] | [[Category: Dosage compensation]] | ||
[[Category: Gene regulation]] | [[Category: Gene regulation]] | ||
Revision as of 16:07, 9 December 2014
Crystal structure of the MSL1-MSL2 complex (3.5A)
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