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[[Image:4es9.png|left|200px]]
==Crystal Structure of the adhesin domain of Epf from Streptococcus pyogenes in P21==
<StructureSection load='4es9' size='340' side='right' caption='[[4es9]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4es9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes_m49_591 Streptococcus pyogenes m49 591]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ES9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ES9 FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4es8|4es8]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SpyoM01000212 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=294934 Streptococcus pyogenes M49 591])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4es9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4es9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4es9 RCSB], [http://www.ebi.ac.uk/pdbsum/4es9 PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Streptococcus pyogenes is an exclusively human pathogen. Streptococcal attachment to and entry into epithelial cells is a prerequisite for a successful infection of the human host and requires adhesins. Here, we demonstrate that the multidomain protein Epf from S. pyogenes serotype M49 is a streptococcal adhesin. An epf--deficient mutant showed significantly decreased adhesion to and internalisation into human keratinocytes. Cell adhesion is mediated by the N-terminal domain of Epf (EpfN) and increased by the human plasma protein plasminogen. The crystal structure of EpfN, solved at 1.6 A resolution, shows that it consists of two subdomains, a carbohydrate-binding module and a fibronectin type III domain. Both fold types commonly participate in ligand-receptor and protein-protein interactions. EpfN is followed by 18 repeats of a domain classified as Domain of Unknown Function 1542 (DUF1542) and a C-terminal cell wall sorting signal. The DUF1542 repeats are not involved in adhesion, but biophysical studies show they are predominantly alpha-helical and form a fibre-like stalk of tandem DUF1542 domains. Epf thus conforms with the widespread family of adhesins known as MSCRAMMs (microbial surface components recognizing adhesive matrix molecules), in which a cell wall-attached stalk enables long-range interactions via its adhesive N-terminal domain.


{{STRUCTURE_4es9|  PDB=4es9  |  SCENE=  }}
The extracellular protein factor Epf from streptococcus pyogenes is a cell-surface adhesin that binds to cells through an N-terminal domain containing a carbohydrate-binding module.,Linke C, Siemens N, Oehmcke S, Radjainia M, Law RH, Whisstock JC, Baker EN, Kreikemeyer B J Biol Chem. 2012 Sep 12. PMID:22977243<ref>PMID:22977243</ref>


===Crystal Structure of the adhesin domain of Epf from Streptococcus pyogenes in P21===
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
{{ABSTRACT_PUBMED_22977243}}
== References ==
 
<references/>
==About this Structure==
__TOC__
[[4es9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes_m49_591 Streptococcus pyogenes m49 591]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ES9 OCA].
</StructureSection>
[[Category: Streptococcus pyogenes m49 591]]
[[Category: Streptococcus pyogenes m49 591]]
[[Category: Baker, E N.]]
[[Category: Baker, E N]]
[[Category: Kreikemeyer, B.]]
[[Category: Kreikemeyer, B]]
[[Category: Linke, C.]]
[[Category: Linke, C]]
[[Category: Siemens, N.]]
[[Category: Siemens, N]]
[[Category: Carbohydrate-binding module]]
[[Category: Carbohydrate-binding module]]
[[Category: Cell adhesion]]
[[Category: Cell adhesion]]
[[Category: Fibronectin-like domain]]
[[Category: Fibronectin-like domain]]
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