4drw: Difference between revisions
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[[ | ==Crystal Structure of the Ternary Complex between S100A10, an Annexin A2 N-terminal Peptide and an AHNAK Peptide== | ||
<StructureSection load='4drw' size='340' side='right' caption='[[4drw]], [[Resolution|resolution]] 3.50Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4drw]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DRW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DRW FirstGlance]. <br> | |||
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">S100A10, ANX2LG, CAL1L, CLP11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4drw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4drw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4drw RCSB], [http://www.ebi.ac.uk/pdbsum/4drw PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Plasma membrane repair involves the coordinated effort of proteins and the inner phospholipid surface to mend the rupture and return the cell back to homeostasis. Here, we present the three-dimensional structure of a multiprotein complex that includes S100A10, annexin A2, and AHNAK, which along with dysferlin, functions in muscle and cardiac tissue repair. The 3.5 A resolution X-ray structure shows that a single region from the AHNAK C terminus is recruited by an S100A10-annexin A2 heterotetramer, forming an asymmetric ternary complex. The AHNAK peptide adopts a coil conformation that arches across the heterotetramer contacting both annexin A2 and S100A10 protomers with tight affinity ( approximately 30 nM) and establishing a structural rationale whereby both S100A10 and annexin proteins are needed in AHNAK recruitment. The structure evokes a model whereby AHNAK is targeted to the membrane surface through sandwiching of the binding region between the S100A10/annexin A2 complex and the phospholipid membrane. | |||
Structure of an asymmetric ternary protein complex provides insight for membrane interaction.,Dempsey BR, Rezvanpour A, Lee TW, Barber KR, Junop MS, Shaw GS Structure. 2012 Oct 10;20(10):1737-45. doi: 10.1016/j.str.2012.08.004. Epub 2012 , Aug 30. PMID:22940583<ref>PMID:22940583</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | |||
*[[S100 protein|S100 protein]] | |||
== | == References == | ||
[[ | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Junop, M S | [[Category: Junop, M S]] | ||
[[Category: Lee, T W | [[Category: Lee, T W]] | ||
[[Category: Rezvanpour, A | [[Category: Rezvanpour, A]] | ||
[[Category: Shaw, G S | [[Category: Shaw, G S]] | ||
[[Category: Atypical ef-hand]] | [[Category: Atypical ef-hand]] | ||
[[Category: Exocytosis-protein binding complex]] | [[Category: Exocytosis-protein binding complex]] | ||
[[Category: Heteropentameric complex]] | [[Category: Heteropentameric complex]] | ||
[[Category: Membrane repair]] | [[Category: Membrane repair]] | ||
