3u30: Difference between revisions
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[[ | ==Crystal structure of a linear-specific Ubiquitin fab bound to linear ubiquitin== | ||
<StructureSection load='3u30' size='340' side='right' caption='[[3u30]], [[Resolution|resolution]] 2.43Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3u30]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U30 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3U30 FirstGlance]. <br> | |||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ubq|1ubq]], [[3dvg|3dvg]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">UBC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3u30 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u30 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3u30 RCSB], [http://www.ebi.ac.uk/pdbsum/3u30 PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Polyubiquitination is an essential posttranslational modification that plays critical roles in cellular signaling. PolyUb (polyubiquitin) chains are formed by linking the carboxyl-terminus of one Ub (ubiquitin) subunit to either a lysine residue or the amino-terminus of an adjacent Ub. Linkage through the amino-terminus results in linear polyubiquitination that has recently been demonstrated to be a key step in nuclear factor kappaB activation; however, tools to study linear chains have been lacking. We therefore engineered a linear-linkage-specific antibody that is functional in Western blot, immunoprecipitation, and immunofluorescence applications. A crystal structure of the linear-linkage-specific antibody Fab fragment in complex with linear diubiquitin provides molecular insight into the nature of linear chain specificity. We use the antibody to demonstrate that linear polyUb is up-regulated upon tumor necrosis factor alpha stimulation of cells, consistent with a critical role in nuclear factor kappaB signaling. This antibody provides an essential tool for further investigation of the function of linear chains. | |||
Engineering and structural characterization of a linear polyubiquitin-specific antibody.,Matsumoto ML, Dong KC, Yu C, Phu L, Gao X, Hannoush RN, Hymowitz SG, Kirkpatrick DS, Dixit VM, Kelley RF J Mol Biol. 2012 May 4;418(3-4):134-44. doi: 10.1016/j.jmb.2011.12.053. Epub 2011, Dec 29. PMID:22227388<ref>PMID:22227388</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | |||
*[[Ubiquitin|Ubiquitin]] | |||
== | == References == | ||
[[ | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Dixit, V M | [[Category: Dixit, V M]] | ||
[[Category: Dong, K C | [[Category: Dong, K C]] | ||
[[Category: Gao, X | [[Category: Gao, X]] | ||
[[Category: Hannoush, R N | [[Category: Hannoush, R N]] | ||
[[Category: Hymowitz, S G | [[Category: Hymowitz, S G]] | ||
[[Category: Kelley, R F | [[Category: Kelley, R F]] | ||
[[Category: Kirkpatrick, D S | [[Category: Kirkpatrick, D S]] | ||
[[Category: Matsumoto, M L | [[Category: Matsumoto, M L]] | ||
[[Category: Phu,L]] | [[Category: Phu,L]] | ||
[[Category: Yu, C | [[Category: Yu, C]] | ||
[[Category: Immune system]] | [[Category: Immune system]] | ||
Revision as of 16:17, 9 December 2014
Crystal structure of a linear-specific Ubiquitin fab bound to linear ubiquitin
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