2bof: Difference between revisions
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==Overview== | ==Overview== | ||
Endoglucanase Cel6A from Thermobifida fusca hydrolyzes the beta-1,4, linkages in cellulose at accessible points along the polymer. The, structure of the catalytic domain of Cel6A from T. fusca in complex with a, nonhydrolysable substrate analogue that acts as an inhibitor, methylcellobiosyl-4-thio-beta-cellobioside (Glc(2)-S-Glc(2)), has been, determined to 1.5 A resolution. The glycosyl unit in subsite -1 was, sterically hindered by Tyr73 and forced into a distorted (2)S(o), conformation. In the enzyme where Tyr73 was mutated to a serine residue, the hindrance was removed and the glycosyl unit in subsite -1 had a, relaxed (4)C(1) chair conformation. The relaxed conformation was seen in, two complex structures of the mutated enzyme, with cellotetrose (Glc(4)), at 1.64 A and .. | Endoglucanase Cel6A from Thermobifida fusca hydrolyzes the beta-1,4, linkages in cellulose at accessible points along the polymer. The, structure of the catalytic domain of Cel6A from T. fusca in complex with a, nonhydrolysable substrate analogue that acts as an inhibitor, methylcellobiosyl-4-thio-beta-cellobioside (Glc(2)-S-Glc(2)), has been, determined to 1.5 A resolution. The glycosyl unit in subsite -1 was, sterically hindered by Tyr73 and forced into a distorted (2)S(o), conformation. In the enzyme where Tyr73 was mutated to a serine residue, the hindrance was removed and the glycosyl unit in subsite -1 had a, relaxed (4)C(1) chair conformation. The relaxed conformation was seen in, two complex structures of the mutated enzyme, with cellotetrose (Glc(4)), at 1.64 A and Glc(2)-S-Glc(2) at 1.04 A resolution. | ||
==About this Structure== | ==About this Structure== | ||
2BOF is a | 2BOF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermobifida_fusca Thermobifida fusca]. Active as [http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BOF OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: tim a/b fold]] | [[Category: tim a/b fold]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 12:26:05 2007'' | ||
