4dns: Difference between revisions
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[[ | ==Crystal structure of Bermuda grass isoallergen BG60 provides insight into the various cross-allergenicity of the pollen group 4 allergens== | ||
<StructureSection load='4dns' size='340' side='right' caption='[[4dns]], [[Resolution|resolution]] 2.15Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4dns]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cynodon_dactylon Cynodon dactylon]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DNS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DNS FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dns OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dns RCSB], [http://www.ebi.ac.uk/pdbsum/4dns PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The structure of Cyn d 4, the group 4 allergen from Bermuda grass, is reported at 2.15 A resolution and is the first crystal structure of a naturally isolated pollen allergen. A conserved N-terminal segment that is only present in the large isoallergens forms extensive interactions with surrounding residues and hence greatly enhances the structural stability of the protein. Cyn d 4 contains an FAD cofactor that is covalently linked to His88 and Cys152. To date, all identified bicovalent flavoproteins are oxidases and their substrates are either sugars or secondary metabolites. A deep large hydrophobic substrate-binding cleft is present. Thus, Cyn d 4 may be an oxidase that is involved in the biosynthesis of a pollen-specific metabolite. Cyn d 4 shares ~70% sequence identity with the Pooideae group 4 allergens. Various cross-reactivities between grass pollen group 4 allergens have previously been demonstrated using sera from allergic patients. The protein surface displays an unusually large number of positively charged clusters, reflecting the high pI of ~10. 38 decapeptides that cover the solvent-accessible sequences did not show any significant IgE-binding activity using sera with high Cyn d 4 reactivity from four patients, suggesting that the IgE epitopes of Cyn d 4 are predominantly conformational in nature. Several group 4 structures were then modelled and their potential cross-reactive and species-specific IgE epitopes were proposed. | |||
Various cross-reactivity of the grass pollen group 4 allergens: crystallographic study of the Bermuda grass isoallergen Cyn d 4.,Huang TH, Peng HJ, Su SN, Liaw SH Acta Crystallogr D Biol Crystallogr. 2012 Oct;68(Pt 10):1303-10. Epub 2012 Sep, 13. PMID:22993084<ref>PMID:22993084</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
== | __TOC__ | ||
</StructureSection> | |||
[[Category: Cynodon dactylon]] | [[Category: Cynodon dactylon]] | ||
[[Category: Huang, T H | [[Category: Huang, T H]] | ||
[[Category: Liaw, S H | [[Category: Liaw, S H]] | ||
[[Category: Peng, H J | [[Category: Peng, H J]] | ||
[[Category: Su, S N | [[Category: Su, S N]] | ||
[[Category: Fad binding domain]] | [[Category: Fad binding domain]] | ||
[[Category: Oxidoreductase]] | [[Category: Oxidoreductase]] | ||
