3v3c: Difference between revisions
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[[ | ==Crystal Structure of Chloroplast ATP synthase c-ring from Pisum sativum== | ||
<StructureSection load='3v3c' size='340' side='right' caption='[[3v3c]], [[Resolution|resolution]] 3.40Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3v3c]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Pisum_sativum Pisum sativum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V3C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3V3C FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DGD:DIGALACTOSYL+DIACYL+GLYCEROL+(DGDG)'>DGD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=YT3:YTTRIUM+(III)+ION'>YT3</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v3c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v3c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3v3c RCSB], [http://www.ebi.ac.uk/pdbsum/3v3c PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
A ring of 8-15 identical c-subunits is essential for ion-translocation by the rotary electromotor of the ubiquitous F(O)F(1)-ATPase. Here we present the crystal structure at 3.4A resolution of the c-ring from chloroplasts of a higher plant (Pisum sativum), determined using a native preparation. The crystal structure was found to resemble that of an (ancestral) cyanobacterium. Using elastic network modeling to investigate the ring's eigen-modes, we found five dominant modes of motion that fell into three classes. They revealed the following deformations of the ring: (I) ellipsoidal, (II) opposite twisting of the luminal circular surface of the ring against the stromal surface, and (III) kinking of the hairpin-shaped monomers in the middle, resulting in bending/stretching of the ring. Extension of the elastic network analysis to rings of different c(n)-symmetry revealed the same classes of dominant modes as in P. sativum (c(14)). We suggest the following functional roles for these classes: The first and third classes of modes affect the interaction of the c-ring with its counterparts in F(O), namely subunits a and bb'. These modes are likely to be involved in ion-translocation and torque generation. The second class of deformation, along with deformations of subunits gamma and epsilon might serve to elastically buffer the torque transmission between F(O) and F(1). | |||
Structure and flexibility of the C-ring in the electromotor of rotary F(o)F(1)-ATPase of pea chloroplasts.,Saroussi S, Schushan M, Ben-Tal N, Junge W, Nelson N PLoS One. 2012;7(9):e43045. doi: 10.1371/journal.pone.0043045. Epub 2012 Sep 25. PMID:23049735<ref>PMID:23049735</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | |||
*[[ATPase|ATPase]] | |||
== | == References == | ||
[[ | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Pisum sativum]] | [[Category: Pisum sativum]] | ||
[[Category: Nelson, N | [[Category: Nelson, N]] | ||
[[Category: Saroussi, S | [[Category: Saroussi, S]] | ||
[[Category: C-ring]] | [[Category: C-ring]] | ||
[[Category: Photosynthesis]] | [[Category: Photosynthesis]] | ||
[[Category: Proton binding]] | [[Category: Proton binding]] | ||
[[Category: Proton translocation]] | [[Category: Proton translocation]] | ||
