4gzz: Difference between revisions

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[[Image:4gzz.jpg|left|200px]]
==Crystal structures of bacterial RNA Polymerase paused elongation complexes==
<StructureSection load='4gzz' size='340' side='right' caption='[[4gzz]], [[Resolution|resolution]] 4.29&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4gzz]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus_hb8 Thermus thermophilus hb8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GZZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GZZ FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rpoA, TTHA1664 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 Thermus thermophilus HB8]), rpoB, TTHA1813 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 Thermus thermophilus HB8]), rpoC, TTHA1812 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 Thermus thermophilus HB8]), rpoZ, TTHA1561 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 Thermus thermophilus HB8])</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gzz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gzz RCSB], [http://www.ebi.ac.uk/pdbsum/4gzz PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Transcriptional pausing by multisubunit RNA polymerases (RNAPs) is a key mechanism for regulating gene expression in both prokaryotes and eukaryotes and is a prerequisite for transcription termination. Pausing and termination states are thought to arise through a common, elemental pause state that is inhibitory for nucleotide addition. We report three crystal structures of Thermus RNAP elemental paused elongation complexes (ePECs). The structures reveal the same relaxed, open-clamp RNAP conformation in the ePEC that may arise by failure to re-establish DNA contacts during translocation. A kinked bridge-helix sterically blocks the RNAP active site, explaining how this conformation inhibits RNAP catalytic activity. Our results provide a framework for understanding how RNA hairpin formation stabilizes the paused state and how the ePEC intermediate facilitates termination.


{{STRUCTURE_4gzz|  PDB=4gzz  |  SCENE=  }}
Structural basis of transcriptional pausing in bacteria.,Weixlbaumer A, Leon K, Landick R, Darst SA Cell. 2013 Jan 31;152(3):431-41. doi: 10.1016/j.cell.2012.12.020. PMID:23374340<ref>PMID:23374340</ref>


===Crystal structures of bacterial RNA Polymerase paused elongation complexes===
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>


{{ABSTRACT_PUBMED_23374340}}
==See Also==
 
*[[RNA polymerase|RNA polymerase]]
==About this Structure==
== References ==
[[4gzz]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus_hb8 Thermus thermophilus hb8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GZZ OCA].
<references/>
__TOC__
</StructureSection>
[[Category: DNA-directed RNA polymerase]]
[[Category: DNA-directed RNA polymerase]]
[[Category: Thermus thermophilus hb8]]
[[Category: Thermus thermophilus hb8]]
[[Category: Darst, S A.]]
[[Category: Darst, S A]]
[[Category: Landick, R.]]
[[Category: Landick, R]]
[[Category: Leon, K.]]
[[Category: Leon, K]]
[[Category: Weixlbaumer, A.]]
[[Category: Weixlbaumer, A]]
[[Category: Dna directed rna transcriptions']]
[[Category: Dna directed rna transcriptions']]
[[Category: Paused transcription elongation complex]]
[[Category: Paused transcription elongation complex]]
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