4hpp: Difference between revisions
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[[ | ==Crystal structure of novel glutamine synthase homolog== | ||
<StructureSection load='4hpp' size='340' side='right' caption='[[4hpp]], [[Resolution|resolution]] 2.50Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4hpp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HPP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HPP FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLU:GLUTAMIC+ACID'>GLU</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PA5508 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 Pseudomonas aeruginosa PAO1])</td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutamate--putrescine_ligase Glutamate--putrescine ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.1.11 6.3.1.11] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hpp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hpp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hpp RCSB], [http://www.ebi.ac.uk/pdbsum/4hpp PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The structure of PA5508 from Pseudomonas aeruginosa, a glutamine synthetase (GS) homologue, has been determined at 2.5 A. Surprisingly, PA5508 forms single hexameric rings rather than the stacked double rings that are characteristic of GS. The C-terminal helical thong motif that links GS rings is present in PA5508; however, it is folded back toward the core of its own polypeptide, preventing it from interacting with a second ring. Interestingly, PA5508 displays a clear preference for aromatic amine substrates. Unique aspects of the structure illustrate how the enzyme is able to catalyze reactions involving bulky amines rather than ammonia. | |||
Structure and Activity of PA5508, a Hexameric Glutamine Synthetase Homologue.,Ladner JE, Atanasova V, Dolezelova Z, Parsons JF Biochemistry. 2012 Dec 21;51(51):10121-3. doi: 10.1021/bi3014856. Epub 2012 Dec, 12. PMID:23234431<ref>PMID:23234431</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
== | __TOC__ | ||
</StructureSection> | |||
[[Category: Glutamate--putrescine ligase]] | [[Category: Glutamate--putrescine ligase]] | ||
[[Category: Pseudomonas aeruginosa pao1]] | [[Category: Pseudomonas aeruginosa pao1]] | ||
[[Category: Atanasova, V | [[Category: Atanasova, V]] | ||
[[Category: Dolezelova, Z | [[Category: Dolezelova, Z]] | ||
[[Category: Ladner, J E | [[Category: Ladner, J E]] | ||
[[Category: Parsons, J F | [[Category: Parsons, J F]] | ||
[[Category: Glutamate]] | [[Category: Glutamate]] | ||
[[Category: Glutamine synthase homolog]] | [[Category: Glutamine synthase homolog]] | ||
[[Category: Ligase]] | [[Category: Ligase]] | ||
[[Category: Polyamine]] | [[Category: Polyamine]] | ||
Revision as of 09:41, 10 December 2014
Crystal structure of novel glutamine synthase homolog
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