4glk: Difference between revisions
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[[ | ==Structure and activity of AbiQ, a lactococcal anti-phage endoribonuclease belonging to the type-III toxin-antitoxin system== | ||
<StructureSection load='4glk' size='340' side='right' caption='[[4glk]], [[Resolution|resolution]] 2.16Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4glk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GLK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GLK FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">abiQ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1358 Lactococcus lactis])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4glk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4glk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4glk RCSB], [http://www.ebi.ac.uk/pdbsum/4glk PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
AbiQ is a phage resistance mechanism found on a native plasmid of Lactococcus lactis that abort virulent phage infections. In this study, we experimentally demonstrate that AbiQ belongs to the recently described type III toxin-antitoxin systems. When overexpressed, the AbiQ protein (ABIQ) is toxic and causes bacterial death in a bacteriostatic manner. Northern and Western blot experiments revealed that the abiQ gene is transcribed and translated constitutively, and its expression is not activated by a phage product. ABIQ is an endoribonuclease that specifically cleaves its cognate antitoxin RNA molecule in vivo. The crystal structure of ABIQ was solved and site-directed mutagenesis identified key amino acids for its anti-phage and/or its RNase function. The AbiQ system is the first lactococcal abortive infection system characterized to date at a structural level. | |||
Structure and activity of AbiQ, a lactococcal endoribonuclease belonging to the type III toxin-antitoxin system.,Samson JE, Spinelli S, Cambillau C, Moineau S Mol Microbiol. 2012 Dec 20. doi: 10.1111/mmi.12129. PMID:23279123<ref>PMID:23279123</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
== | __TOC__ | ||
</StructureSection> | |||
[[Category: Lactococcus lactis]] | [[Category: Lactococcus lactis]] | ||
[[Category: Cambillau, C | [[Category: Cambillau, C]] | ||
[[Category: Moineau, S | [[Category: Moineau, S]] | ||
[[Category: Samson, J | [[Category: Samson, J]] | ||
[[Category: Spinelli, S | [[Category: Spinelli, S]] | ||
[[Category: Alpha beta]] | [[Category: Alpha beta]] | ||
[[Category: Endonuclease]] | [[Category: Endonuclease]] | ||
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
[[Category: Rna antiq]] | [[Category: Rna antiq]] | ||
Revision as of 09:45, 10 December 2014
Structure and activity of AbiQ, a lactococcal anti-phage endoribonuclease belonging to the type-III toxin-antitoxin system
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