1wcm: Difference between revisions

We determined the x-ray structure of the RNA polymerase (Pol) II, subcomplex Rpb4/7 at 2.3 A resolution, combined it with a previous, structure of the 10-subunit polymerase core, and refined an atomic model, of the complete 12-subunit Pol II at 3.8-A resolution. Comparison of the, complete Pol II structure with structures of the Pol II core and free, Rpb4/7 shows that the core-Rpb4/7 interaction goes along with formation of, an alpha-helix in the linker region of the largest Pol II subunit and with, folding of the conserved Rpb7 tip loop. Details of the core-Rpb4/7, interface explain facilitated Rpb4/7 dissociation in a, temperature-sensitive Pol II mutant and specific assembly of Pol I with, its Rpb4/7 counterpart, A43/14. The refined atomic model of Pol II serves, as the new reference ... [[http://ispc.weizmann.ac.il/pmbin/getpm?15591044 (full description)]]

1WCM is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]] with ZN and MG as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WCM OCA]].  

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