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| ==Real space refined coordinates of the 30S subunit fitted into the low resolution cryo-EM map of the EF-G.GTP state of E. coli 70S ribosome==
| | #REDIRECT [[4v47]] This PDB entry is obsolete and replaced by 4v47 |
| <StructureSection load='1p6g' size='340' side='right' caption='[[1p6g]], [[Resolution|resolution]] 12.30Å' scene=''>
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| == Structural highlights ==
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| <table><tr><td colspan='2'>[[1p6g]] is a 19 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P6G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1P6G FirstGlance]. <br>
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| </td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1gix|1gix]], [[1giy|1giy]], [[1jj2|1jj2]], [[1ibl|1ibl]], [[1lnr|1lnr]], [[1m5g|1m5g]], [[1p85|1p85]]</td></tr>
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| <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1p6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p6g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1p6g RCSB], [http://www.ebi.ac.uk/pdbsum/1p6g PDBsum]</span></td></tr>
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| <table>
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| == Evolutionary Conservation ==
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| [[Image:Consurf_key_small.gif|200px|right]]
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| Check<jmol>
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| <jmolCheckbox>
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| <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p6/1p6g_consurf.spt"</scriptWhenChecked>
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| <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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| <text>to colour the structure by Evolutionary Conservation</text>
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| </jmolCheckbox>
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| </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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| <div style="clear:both"></div>
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| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| Cryo-EM density maps showing the 70S ribosome of E. coli in two different functional states related by a ratchet-like motion were analyzed using real-space refinement. Comparison of the two resulting atomic models shows that the ribosome changes from a compact structure to a looser one, coupled with the rearrangement of many of the proteins. Furthermore, in contrast to the unchanged inter-subunit bridges formed wholly by RNA, the bridges involving proteins undergo large conformational changes following the ratchet-like motion, suggesting an important role of ribosomal proteins in facilitating the dynamics of translation.
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| Study of the structural dynamics of the E coli 70S ribosome using real-space refinement.,Gao H, Sengupta J, Valle M, Korostelev A, Eswar N, Stagg SM, Van Roey P, Agrawal RK, Harvey SC, Sali A, Chapman MS, Frank J Cell. 2003 Jun 13;113(6):789-801. PMID:12809609<ref>PMID:12809609</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| == References ==
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| <references/>
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| __TOC__
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| </StructureSection>
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| [[Category: Escherichia coli]]
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| [[Category: Agrawal, R K.]]
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| [[Category: Chapman, M S.]]
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| [[Category: Eswar, N.]]
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| [[Category: Frank, J.]]
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| [[Category: Gao, H.]]
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| [[Category: Harvey, S T.]]
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| [[Category: Korostelev, A]]
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| [[Category: Roey, P Van.]]
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| [[Category: Sali, A.]]
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| [[Category: Sengupta, J.]]
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| [[Category: Stagg, S M.]]
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| [[Category: Valle, M.]]
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| [[Category: Efg-gtp-bound state]]
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| [[Category: Ratchet-like movement]]
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| [[Category: Real-space refinement]]
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| [[Category: Ribosome]]
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