|
|
| Line 1: |
Line 1: |
| ==THE STRUCTURE OF THE RIBOSOME WITH ELONGATION FACTOR G TRAPPED IN THE POST-TRANSLOCATIONAL STATE (PART 1 OF 4).==
| | #REDIRECT [[4v5f]] This PDB entry is obsolete and replaced by 4v5f |
| <StructureSection load='2wri' size='340' side='right' caption='[[2wri]], [[Resolution|resolution]] 3.60Å' scene=''>
| |
| == Structural highlights ==
| |
| <table><tr><td colspan='2'>[[2wri]] is a 25 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. The January 2010 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''70S Ribosomes'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2010_1 10.2210/rcsb_pdb/mom_2010_1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WRI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WRI FirstGlance]. <br>
| |
| </td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FUA:FUSIDIC+ACID'>FUA</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
| |
| <tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene></td></tr>
| |
| <tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2wrj|2wrj]], [[2wrk|2wrk]], [[2wrl|2wrl]], [[1ibk|1ibk]], [[1ibl|1ibl]], [[2uuc|2uuc]], [[2wdk|2wdk]], [[2j02|2j02]], [[1j5e|1j5e]], [[2j00|2j00]], [[1hnx|1hnx]], [[2b9o|2b9o]], [[2wh3|2wh3]], [[2uub|2uub]], [[1vov|1vov]], [[1emi|1emi]], [[1pnx|1pnx]], [[2v46|2v46]], [[2efg|2efg]], [[2vqf|2vqf]], [[2wh1|2wh1]], [[1n32|1n32]], [[1elo|1elo]], [[2f4v|2f4v]], [[1twt|1twt]], [[1qzc|1qzc]], [[2uxd|2uxd]], [[1fka|1fka]], [[1fjg|1fjg]], [[1n36|1n36]], [[2uxc|2uxc]], [[1hnw|1hnw]], [[1l1u|1l1u]], [[1hr0|1hr0]], [[2v48|2v48]], [[2b64|2b64]], [[1xmq|1xmq]], [[1dar|1dar]], [[2b9m|2b9m]], [[1pn8|1pn8]], [[2wdg|2wdg]], [[1xnr|1xnr]], [[1hnz|1hnz]], [[1dv4|1dv4]], [[1rss|1rss]], [[2wdh|2wdh]], [[1jgq|1jgq]], [[1i94|1i94]], [[1i96|1i96]], [[2uu9|2uu9]], [[1pn7|1pn7]], [[1eg0|1eg0]], [[1ibm|1ibm]], [[2uxb|2uxb]], [[2vqe|2vqe]], [[1g1x|1g1x]], [[2uua|2uua]], [[1i95|1i95]], [[1xnq|1xnq]], [[1qd7|1qd7]], [[1gix|1gix]], [[1efg|1efg]], [[1jgo|1jgo]], [[1jgp|1jgp]], [[1n34|1n34]], [[1n33|1n33]], [[1pns|1pns]], [[1yl4|1yl4]], [[2wdm|2wdm]], [[1xmo|1xmo]], [[1i97|1i97]], [[2w2n|2w2n]], [[2wrr|2wrr]], [[2wrq|2wrq]], [[2wro|2wro]], [[2wrn|2wrn]]</td></tr>
| |
| <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wri FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wri OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wri RCSB], [http://www.ebi.ac.uk/pdbsum/2wri PDBsum]</span></td></tr>
| |
| <table>
| |
| == Evolutionary Conservation ==
| |
| [[Image:Consurf_key_small.gif|200px|right]]
| |
| Check<jmol>
| |
| <jmolCheckbox>
| |
| <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wr/2wri_consurf.spt"</scriptWhenChecked>
| |
| <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
| |
| <text>to colour the structure by Evolutionary Conservation</text>
| |
| </jmolCheckbox>
| |
| </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
| |
| <div style="clear:both"></div>
| |
| <div style="background-color:#fffaf0;">
| |
| == Publication Abstract from PubMed ==
| |
| Elongation factor G (EF-G) is a guanosine triphosphatase (GTPase) that plays a crucial role in the translocation of transfer RNAs (tRNAs) and messenger RNA (mRNA) during translation by the ribosome. We report a crystal structure refined to 3.6 angstrom resolution of the ribosome trapped with EF-G in the posttranslocational state using the antibiotic fusidic acid. Fusidic acid traps EF-G in a conformation intermediate between the guanosine triphosphate and guanosine diphosphate forms. The interaction of EF-G with ribosomal elements implicated in stimulating catalysis, such as the L10-L12 stalk and the L11 region, and of domain IV of EF-G with the tRNA at the peptidyl-tRNA binding site (P site) and with mRNA shed light on the role of these elements in EF-G function. The stabilization of the mobile stalks of the ribosome also results in a more complete description of its structure.
| |
| | |
| The structure of the ribosome with elongation factor G trapped in the posttranslocational state.,Gao YG, Selmer M, Dunham CM, Weixlbaumer A, Kelley AC, Ramakrishnan V Science. 2009 Oct 30;326(5953):694-9. PMID:19833919<ref>PMID:19833919</ref>
| |
| | |
| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| |
| </div>
| |
| | |
| ==See Also==
| |
| *[[Elongation factor|Elongation factor]]
| |
| *[[Escherichia coli LepA%2C the ribosomal back translocase|Escherichia coli LepA%2C the ribosomal back translocase]]
| |
| *[[Ribosomal protein THX|Ribosomal protein THX]]
| |
| *[[Ribosome 3D structures|Ribosome 3D structures]]
| |
| == References ==
| |
| <references/>
| |
| __TOC__
| |
| </StructureSection>
| |
| [[Category: 70S Ribosomes]]
| |
| [[Category: Escherichia coli]]
| |
| [[Category: RCSB PDB Molecule of the Month]]
| |
| [[Category: Thermus thermophilus]]
| |
| [[Category: Dunham, C M.]]
| |
| [[Category: Gao, Y G.]]
| |
| [[Category: Kelley, A C.]]
| |
| [[Category: Ramakrishnan, V.]]
| |
| [[Category: Selmer, M.]]
| |
| [[Category: Weixlbaumer, A.]]
| |
| [[Category: Elongation factor]]
| |
| [[Category: Elongation factor g]]
| |
| [[Category: Metal-binding]]
| |
| [[Category: Nucleotide-binding]]
| |
| [[Category: Protein biosynthesis]]
| |
| [[Category: Protein synthesis]]
| |
| [[Category: Ribonucleoprotein]]
| |
| [[Category: Ribosomal protein]]
| |
| [[Category: Ribosome]]
| |
| [[Category: Rna-binding]]
| |
| [[Category: Rrna-binding]]
| |
| [[Category: Translation]]
| |
| [[Category: Translocation]]
| |
| [[Category: Trna-binding]]
| |
| [[Category: Zinc-finger]]
| |
This PDB entry is obsolete and replaced by 4v5f
