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| ==Elongation complex of the 70S ribosome with three tRNAs and mRNA. This entry 3I8G contains 30S ribosomal subnit.The 50S ribosomal subunit can be found in PDB entry 3I8F. Molecule B in the same asymmetric unit is deposited as 3I8G (30S) and 3I8F (50S).==
| | #REDIRECT [[4v6f]] This PDB entry is obsolete and replaced by 4v6f |
| <StructureSection load='3i8g' size='340' side='right' caption='[[3i8g]], [[Resolution|resolution]] 3.10Å' scene=''>
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| == Structural highlights ==
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| <table><tr><td colspan='2'>[[3i8g]] is a 25 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I8G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3I8G FirstGlance]. <br>
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| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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| <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MIA:2-METHYLTHIO-N6-ISOPENTENYL-ADENOSINE-5-MONOPHOSPHATE'>MIA</scene></td></tr>
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| <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3i8f|3i8f]], [[3i8h|3i8h]], [[3i8i|3i8i]]</td></tr>
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| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i8g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i8g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3i8g RCSB], [http://www.ebi.ac.uk/pdbsum/3i8g PDBsum]</span></td></tr>
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| </table>
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| == Evolutionary Conservation ==
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| [[Image:Consurf_key_small.gif|200px|right]]
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| Check<jmol>
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| <jmolCheckbox>
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| <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i8/3i8g_consurf.spt"</scriptWhenChecked>
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| <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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| <text>to colour the structure by Evolutionary Conservation</text>
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| </jmolCheckbox>
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| </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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| <div style="clear:both"></div>
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| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| One key question in protein biosynthesis is how the ribosome couples mRNA and tRNA movements to prevent disruption of weak codon-anticodon interactions and loss of the translational reading frame during translocation. Here we report the complete path of mRNA on the 70S ribosome at the atomic level (3.1-A resolution), and we show that one of the conformational rearrangements that occurs upon transition from initiation to elongation is a narrowing of the downstream mRNA tunnel. This rearrangement triggers formation of a network of interactions between the mRNA downstream of the A-site codon and the elongating ribosome. Our data elucidate the mechanism by which hypermodified nucleoside 2-methylthio-N6 isopentenyl adenosine at position 37 (ms(2)i(6)A37) in tRNA(Phe)(GAA) stabilizes mRNA-tRNA interactions in all three tRNA binding sites. Another network of contacts is formed between this tRNA modification and ribosomal elements surrounding the mRNA E/P kink, resulting in the anchoring of P-site tRNA. These data allow rationalization of how modification deficiencies of ms(2)i(6)A37 in tRNAs may lead to shifts of the translational reading frame.
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| Structural aspects of messenger RNA reading frame maintenance by the ribosome.,Jenner LB, Demeshkina N, Yusupova G, Yusupov M Nat Struct Mol Biol. 2010 May;17(5):555-60. Epub 2010 Apr 18. PMID:20400952<ref>PMID:20400952</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| ==See Also==
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| *[[Ribosomal protein THX|Ribosomal protein THX]]
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| *[[Ribosome 3D structures|Ribosome 3D structures]]
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| == References ==
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| <references/>
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| __TOC__
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| </StructureSection>
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| [[Category: Escherichia coli]]
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| [[Category: Thermus thermophilus]]
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| [[Category: Jenner, L B]]
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| [[Category: Yusupov, M]]
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| [[Category: Yusupova, G]]
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| [[Category: 16s ribosomal rna]]
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| [[Category: Metal-binding]]
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| [[Category: Mrna]]
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| [[Category: Ribonucleoprotein]]
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| [[Category: Ribosomal protein]]
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| [[Category: Ribosomal rna]]
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| [[Category: Ribosome]]
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| [[Category: Rna-binding]]
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| [[Category: Rrna-binding]]
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| [[Category: Trna]]
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| [[Category: Trna-binding]]
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| [[Category: Zinc-finger]]
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