|
|
| Line 1: |
Line 1: |
| ==Atomic model of an infectious rotavirus particle==
| | #REDIRECT [[4v7q]] This PDB entry is obsolete and replaced by 4v7q |
| <StructureSection load='3iyu' size='340' side='right' caption='[[3iyu]], [[Resolution|resolution]] 3.80Å' scene=''>
| |
| == Structural highlights ==
| |
| <table><tr><td colspan='2'>[[3iyu]] is a 16 chain structure with sequence from [http://en.wikipedia.org/wiki/Simian_rotavirus_a Simian rotavirus a]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IYU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IYU FirstGlance]. <br>
| |
| </td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene><br>
| |
| <tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3n09|3n09]]</td></tr>
| |
| <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3iyu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iyu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3iyu RCSB], [http://www.ebi.ac.uk/pdbsum/3iyu PDBsum]</span></td></tr>
| |
| <table>
| |
| <div style="background-color:#fffaf0;">
| |
| == Publication Abstract from PubMed ==
| |
| Non-enveloped viruses of different types have evolved distinct mechanisms for penetrating a cellular membrane during infection. Rotavirus penetration appears to occur by a process resembling enveloped-virus fusion: membrane distortion linked to conformational changes in a viral protein. Evidence for such a mechanism comes from crystallographic analyses of fragments of VP4, the rotavirus-penetration protein, and infectivity analyses of structure-based VP4 mutants. We describe here the structure of an infectious rotavirus particle determined by electron cryomicroscopy (cryoEM) and single-particle analysis at about 4.3 A resolution. The cryoEM image reconstruction permits a nearly complete trace of the VP4 polypeptide chain, including the positions of most side chains. It shows how the two subfragments of VP4 (VP8(*) and VP5(*)) retain their association after proteolytic cleavage, reveals multiple structural roles for the beta-barrel domain of VP5(*), and specifies interactions of VP4 with other capsid proteins. The virion model allows us to integrate structural and functional information into a coherent mechanism for rotavirus entry.
| |
| | |
| Atomic model of an infectious rotavirus particle.,Settembre EC, Chen JZ, Dormitzer PR, Grigorieff N, Harrison SC EMBO J. 2011 Jan 19;30(2):408-16. Epub 2010 Dec 14. PMID:21157433<ref>PMID:21157433</ref>
| |
| | |
| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| |
| </div>
| |
| == References ==
| |
| <references/>
| |
| __TOC__
| |
| </StructureSection>
| |
| [[Category: Simian rotavirus a]]
| |
| [[Category: Chen, J Z.]]
| |
| [[Category: Dormitzer, P R.]]
| |
| [[Category: Grigorieff, N.]]
| |
| [[Category: Harrison, S C.]]
| |
| [[Category: Settembre, E C.]]
| |
| [[Category: De novo]]
| |
| [[Category: Double layered particle]]
| |
| [[Category: Icosahedral virus]]
| |
| [[Category: Infectious]]
| |
| [[Category: Near atomic resolution]]
| |
| [[Category: Rotavirus]]
| |
| [[Category: Triple layered particle]]
| |
| [[Category: Virus]]
| |
| [[Category: Vp4]]
| |
| [[Category: Vp7]]
| |
This PDB entry is obsolete and replaced by 4v7q
