1oqz: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1oqz.gif|left|200px]] | [[Image:1oqz.gif|left|200px]] | ||
'''Crystal Structures of Glutaryl 7-Aminocephalosporanic Acid Acylase: Insight into Autoproteolytic Activation''' | {{Structure | ||
|PDB= 1oqz |SIZE=350|CAPTION= <scene name='initialview01'>1oqz</scene>, resolution 2.50Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Crystal Structures of Glutaryl 7-Aminocephalosporanic Acid Acylase: Insight into Autoproteolytic Activation''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
1OQZ is a [ | 1OQZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_sp._se83 Pseudomonas sp. se83]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OQZ OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structures of glutaryl 7-aminocephalosporanic acid acylase: insight into autoproteolytic activation., Kim JK, Yang IS, Rhee S, Dauter Z, Lee YS, Park SS, Kim KH, Biochemistry. 2003 Apr 15;42(14):4084-93. PMID:[http:// | Crystal structures of glutaryl 7-aminocephalosporanic acid acylase: insight into autoproteolytic activation., Kim JK, Yang IS, Rhee S, Dauter Z, Lee YS, Park SS, Kim KH, Biochemistry. 2003 Apr 15;42(14):4084-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12680762 12680762] | ||
[[Category: Pseudomonas sp. se83]] | [[Category: Pseudomonas sp. se83]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 23: | Line 32: | ||
[[Category: glutaryl 7-aminocephalosporanic acid]] | [[Category: glutaryl 7-aminocephalosporanic acid]] | ||
[[Category: glutaryl 7-aminocephalosporanic acid acylase]] | [[Category: glutaryl 7-aminocephalosporanic acid acylase]] | ||
[[Category: n-terminal nucleophile (ntn) | [[Category: n-terminal nucleophile (ntn) hydrolase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:14:25 2008'' | ||
Revision as of 11:14, 20 March 2008
| |||||||||||||
| 1oqz, resolution 2.50Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal Structures of Glutaryl 7-Aminocephalosporanic Acid Acylase: Insight into Autoproteolytic Activation
Overview
Glutaryl 7-aminocephalosporanic acid acylase (GCA, EC 3.5.1.11) is a member of N-terminal nucleophile (Ntn) hydrolases. The native enzyme is an (alpha beta)(2) heterotetramer originated from an enzymatically inactive precursor of a single polypeptide. The activation of precursor GCA consists of primary and secondary autoproteolytic cleavages, generating a terminal residue with both a nucleophile and a base and releasing a nine amino acid spacer peptide. We have determined the crystal structures of the recombinant selenomethionyl native and S170A mutant precursor from Pseudomonas sp. strain GK16. Precursor activation is likely triggered by conformational constraints within the spacer peptide, probably inducing a peptide flip. Autoproteolytic site solvent molecules, which have been trapped in a hydrophobic environment by the spacer peptide, may play a role as a general base for nucleophilic attack. The activation results in building up a catalytic triad composed of Ser170/His192/Glu624. However, the triad is not linked to the usual hydroxyl but the free alpha-amino group of the N-terminal serine residue of the native GCA. Mutagenesis and structural data support the notion that the stabilization of a transient hydroxazolidine ring during autoproteolysis would be critical during the N --> O acyl shift. The autoproteolytic activation mechanism for GCA is described.
About this Structure
1OQZ is a Single protein structure of sequence from Pseudomonas sp. se83. Full crystallographic information is available from OCA.
Reference
Crystal structures of glutaryl 7-aminocephalosporanic acid acylase: insight into autoproteolytic activation., Kim JK, Yang IS, Rhee S, Dauter Z, Lee YS, Park SS, Kim KH, Biochemistry. 2003 Apr 15;42(14):4084-93. PMID:12680762
Page seeded by OCA on Thu Mar 20 13:14:25 2008
