3bnl: Difference between revisions
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==Crystal structure of the bacterial ribosomal decoding A site in the presence of [Co(NH3)6]Cl3== | |||
<StructureSection load='3bnl' size='340' side='right' caption='[[3bnl]], [[Resolution|resolution]] 2.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3bnl]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BNL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BNL FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3bnn|3bnn]], [[3bno|3bno]], [[3bnp|3bnp]], [[3bnq|3bnq]], [[3bnr|3bnr]], [[3bns|3bns]], [[3bnt|3bnt]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bnl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bnl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3bnl RCSB], [http://www.ebi.ac.uk/pdbsum/3bnl PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The A site of the small ribosomal subunit participates in the fidelity of decoding by switching between two states, a resting 'off' state and an active decoding 'on' state. Eight crystal structures of RNA duplexes containing two minimal decoding A sites of the Homo sapiens mitochondrial wild-type, the A1555G mutant or bacteria have been solved. The resting 'off' state of the mitochondrial wild-type A site is surprisingly different from that of the bacterial A site. The mitochondrial A1555G mutant has two types of the 'off' states; one is similar to the mitochondrial wild-type 'off' state and the other is similar to the bacterial 'off' state. Our present results indicate that the dynamics of the A site in bacteria and mitochondria are different, a property probably related to the small number of tRNAs used for decoding in mitochondria. Based on these structures, we propose a hypothesis for the molecular mechanism of non-syndromic hearing loss due to the mitochondrial A1555G mutation. | |||
The bacterial and mitochondrial ribosomal A-site molecular switches possess different conformational substates.,Kondo J, Westhof E Nucleic Acids Res. 2008 May;36(8):2654-66. Epub 2008 Mar 16. PMID:18346970<ref>PMID:18346970</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | ==See Also== | ||
*[[Ribosomal A Site Binding Paromomycin: A Morph|Ribosomal A Site Binding Paromomycin: A Morph]] | *[[Ribosomal A Site Binding Paromomycin: A Morph|Ribosomal A Site Binding Paromomycin: A Morph]] | ||
== References == | |||
== | <references/> | ||
< | __TOC__ | ||
[[Category: Kondo, J | </StructureSection> | ||
[[Category: Westhof, E | [[Category: Kondo, J]] | ||
[[Category: Westhof, E]] | |||
[[Category: Decoding site]] | [[Category: Decoding site]] | ||
[[Category: Ribosome]] | [[Category: Ribosome]] | ||
[[Category: Rna]] | [[Category: Rna]] | ||
