2l1f: Difference between revisions
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==Structure of a conserved retroviral RNA packaging element by NMR spectroscopy and cryo-electron tomography== | |||
<StructureSection load='2l1f' size='340' side='right' caption='[[2l1f]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2l1f]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L1F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L1F FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l1f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l1f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2l1f RCSB], [http://www.ebi.ac.uk/pdbsum/2l1f PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The 5'-untranslated regions of all gammaretroviruses contain a conserved "double-hairpin motif" (Psi(CD)) that is required for genome packaging. Both hairpins (SL-C and SL-D) contain GACG tetraloops that, in isolated RNAs, are capable of forming "kissing" interactions stabilized by two intermolecular G-C base pairs. We have determined the three-dimensional structure of the double hairpin from the Moloney murine leukemia virus ([Psi(CD)](2), 132 nt, 42.8 kDa) using a (2)H-edited NMR-spectroscopy-based approach. This approach enabled the detection of (1)H-(1)H dipolar interactions that were not observed in previous studies of isolated SL-C and SL-D hairpin RNAs using traditional (1)H-(1)H correlated and (1)H-(13)C-edited NMR methods. The hairpins participate in intermolecular cross-kissing interactions (SL-C to SL-D' and SLC' to SL-D) and stack in an end-to-end manner (SL-C to SL-D and SL-C' to SL-D') that gives rise to an elongated overall shape (ca 95 Ax45 Ax25 A). The global structure was confirmed by cryo-electron tomography (cryo-ET), making [Psi(CD)](2) simultaneously the smallest RNA to be structurally characterized to date by cryo-ET and among the largest to be determined by NMR. Our findings suggest that, in addition to promoting dimerization, [Psi(CD)](2) functions as a scaffold that helps initiate virus assembly by exposing a cluster of conserved UCUG elements for binding to the cognate nucleocapsid domains of assembling viral Gag proteins. | |||
Structure of a Conserved Retroviral RNA Packaging Element by NMR Spectroscopy and Cryo-Electron Tomography.,Miyazaki Y, Irobalieva RN, Tolbert B, Smalls-Mantey A, Iyalla K, Loeliger K, D'Souza V, Khant H, Schmid MF, Garcia E, Telesnitsky A, Chiu W, Summers MF J Mol Biol. 2010 Oct 8. PMID:20933521<ref>PMID:20933521</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
< | </div> | ||
[[Category: Chiu, W | == References == | ||
[[Category: Garcia, E | <references/> | ||
[[Category: Irobalieva, R N | __TOC__ | ||
[[Category: Iyalla, K | </StructureSection> | ||
[[Category: Khant, H | [[Category: Chiu, W]] | ||
[[Category: Loeliger, K | [[Category: Garcia, E]] | ||
[[Category: Miyazaki, Y | [[Category: Irobalieva, R N]] | ||
[[Category: Schmid, M | [[Category: Iyalla, K]] | ||
[[Category: Smalls-Manty, A | [[Category: Khant, H]] | ||
[[Category: Souza, V D | [[Category: Loeliger, K]] | ||
[[Category: Summers, M F | [[Category: Miyazaki, Y]] | ||
[[Category: Telesnitsky, A | [[Category: Schmid, M]] | ||
[[Category: Tolbert, B | [[Category: Smalls-Manty, A]] | ||
[[Category: Souza, V D]] | |||
[[Category: Summers, M F]] | |||
[[Category: Telesnitsky, A]] | |||
[[Category: Tolbert, B]] | |||
[[Category: Cryo-et]] | [[Category: Cryo-et]] | ||
[[Category: Packaging]] | [[Category: Packaging]] | ||
[[Category: Retrovirus]] | [[Category: Retrovirus]] | ||
[[Category: Rna]] | [[Category: Rna]] | ||
Revision as of 14:11, 17 December 2014
Structure of a conserved retroviral RNA packaging element by NMR spectroscopy and cryo-electron tomography
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