3mlh: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Crystal structure of the 2009 H1N1 influenza virus hemagglutinin receptor-binding domain== | |||
<StructureSection load='3mlh' size='340' side='right' caption='[[3mlh]], [[Resolution|resolution]] 2.09Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3mlh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Influenza_a_virus Influenza a virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MLH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MLH FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11320 Influenza A virus])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mlh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mlh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3mlh RCSB], [http://www.ebi.ac.uk/pdbsum/3mlh PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The hemagglutinin (HA) surface glycoprotein promotes influenza virus entry and is the key protective antigen in natural immunity and vaccines. The HA protein is a trimeric envelope glycoprotein consisting of a globular receptor-binding domain (HA-RBD) that is inserted into a membrane fusion-mediating stalk domain. Similar to other Class I viral fusion proteins, the fusogenic stalk domain spontaneously refolds into its postfusion conformation when expressed in isolation, consistent with this domain being trapped in a metastable conformation. Using X-ray crystallography, we show that the influenza virus HA-RBD refolds spontaneously into its native, immunogenic structure even when expressed in an unglycosylated form in Escherichia coli. In the 2.10 A structure of the HA-RBD, the receptor-binding pocket is intact and its conformational epitopes are preserved. Along with recombinant HA-RBD being immunogenic and protective in ferrets, the protein binds with specificity to sera from influenza virus-infected patients. Overall, the data provide a structural basis for the rapid production of influenza vaccines in E. coli. From an evolutionary standpoint, the ability of the HA-RBD to refold spontaneously into its native conformation suggests that influenza virus acquired this domain as an insertion into an ancestral membrane-fusion domain. The insertion of independently folding domains into fusogenic stalk domains may be a common feature of Class I viral fusion proteins. | |||
The Receptor-Binding Domain of Influenza Virus Hemagglutinin Produced in Escherichia coli Folds into its Native, Immunogenic Structure.,Dubois RM, Aguilar-Yanez JM, Mendoza-Ochoa GI, Oropeza-Almazan Y, Schultz-Cherry S, Alvarez MM, White SW, Russell CJ J Virol. 2010 Nov 10. PMID:21068239<ref>PMID:21068239</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | ==See Also== | ||
*[[Hemagglutinin|Hemagglutinin]] | *[[Hemagglutinin|Hemagglutinin]] | ||
== References == | |||
== | <references/> | ||
< | __TOC__ | ||
</StructureSection> | |||
[[Category: Influenza a virus]] | [[Category: Influenza a virus]] | ||
[[Category: Aguilar-Yanez, J M | [[Category: Aguilar-Yanez, J M]] | ||
[[Category: Alvarez, M M | [[Category: Alvarez, M M]] | ||
[[Category: DuBois, R M | [[Category: DuBois, R M]] | ||
[[Category: Mendoza-Ochoa, G I | [[Category: Mendoza-Ochoa, G I]] | ||
[[Category: Russell, C J | [[Category: Russell, C J]] | ||
[[Category: Schultz-Cherry, S | [[Category: Schultz-Cherry, S]] | ||
[[Category: White, S W | [[Category: White, S W]] | ||
[[Category: Antigen]] | [[Category: Antigen]] | ||
[[Category: Hemagglutinin]] | [[Category: Hemagglutinin]] | ||
Revision as of 08:38, 18 December 2014
Crystal structure of the 2009 H1N1 influenza virus hemagglutinin receptor-binding domain
| ||||||||||||
