1vt5: Difference between revisions
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==THE CRYSTAL STRUCTURE OF D(CCCCGGGG): A NEW A-FORM VARIANT WITH AN EXTENDED BACKBONE CONFORMATION== | |||
<StructureSection load='1vt5' size='340' side='right' caption='[[1vt5]], [[Resolution|resolution]] 2.25Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1vt5]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VT5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1VT5 FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vt5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vt5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1vt5 RCSB], [http://www.ebi.ac.uk/pdbsum/1vt5 PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The crystal structure of d(CCCCGGGG) has been determined at a resolution of 2.25 A. The oligomers crystallize as A-DNA duplexes occupying crystallographic two-fold axes. The backbone conformation is, in general, similar to that observed in previously reported crystal structures of A-DNA fragments, except for the central linkage, where it adopts an extended structure resulting from all trans conformation at the P-O5'-C5'-C4' bonds. This type of conformation facilitates interstrand stacking between the guanines at the C-G site. The local helix twist at this step is very small (25 degrees) compared to an overall average of 33.5 degrees. The unique structure of the C-G base-pair step, namely the extended backbone and the distinct stacking geometry, may be an important feature in the recognition mechanism between double-stranded DNA molecules and restriction endonucleases such as Msp I, which cuts the sequence CCGG very specifically with a rate unaffected by neighboring base pairs. | |||
The crystal structure of d(CCCCGGGG): a new A-form variant with an extended backbone conformation.,Haran TE, Shakked Z, Wang AH, Rich A J Biomol Struct Dyn. 1987 Oct;5(2):199-217. PMID:3271472<ref>PMID:3271472</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
[[Category: Haran, T E | == References == | ||
[[Category: Rich, A | <references/> | ||
[[Category: Shakked, Z | __TOC__ | ||
[[Category: Wang, A H.J | </StructureSection> | ||
[[Category: Haran, T E]] | |||
[[Category: Rich, A]] | |||
[[Category: Shakked, Z]] | |||
[[Category: Wang, A H.J]] | |||
[[Category: A-dna]] | [[Category: A-dna]] | ||
[[Category: Dna]] | [[Category: Dna]] | ||
[[Category: Double helix]] | [[Category: Double helix]] | ||
Revision as of 11:29, 18 December 2014
THE CRYSTAL STRUCTURE OF D(CCCCGGGG): A NEW A-FORM VARIANT WITH AN EXTENDED BACKBONE CONFORMATION
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