3b31: Difference between revisions
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==Crystal structure of domain III of the Cricket Paralysis Virus IRES RNA== | |||
<StructureSection load='3b31' size='340' side='right' caption='[[3b31]], [[Resolution|resolution]] 2.40Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3b31]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B31 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3B31 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IRI:IRIDIUM+HEXAMMINE+ION'>IRI</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b31 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3b31 RCSB], [http://www.ebi.ac.uk/pdbsum/3b31 PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Internal ribosome entry site (IRES) RNAs initiate protein synthesis in eukaryotic cells by a noncanonical cap-independent mechanism. IRESes are critical for many pathogenic viruses, but efforts to understand their function are complicated by the diversity of IRES sequences as well as by limited high-resolution structural information. The intergenic region (IGR) IRESes of the Dicistroviridae viruses are powerful model systems to begin to understand IRES function. Here we present the crystal structure of a Dicistroviridae IGR IRES domain that interacts with the ribosome's decoding groove. We find that this RNA domain precisely mimics the transfer RNA anticodon-messenger RNA codon interaction, and its modeled orientation on the ribosome helps explain translocation without peptide bond formation. When combined with a previous structure, this work completes the first high-resolution description of an IRES RNA and provides insight into how RNAs can manipulate complex biological machines. | |||
tRNA-mRNA mimicry drives translation initiation from a viral IRES.,Costantino DA, Pfingsten JS, Rambo RP, Kieft JS Nat Struct Mol Biol. 2008 Jan;15(1):57-64. Epub 2007 Dec 23. PMID:18157151<ref>PMID:18157151</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
[[Category: Costantino, D A | == References == | ||
[[Category: Kieft, J S | <references/> | ||
[[Category: Pfingsten, J S | __TOC__ | ||
[[Category: Rambo, R P | </StructureSection> | ||
[[Category: Costantino, D A]] | |||
[[Category: Kieft, J S]] | |||
[[Category: Pfingsten, J S]] | |||
[[Category: Rambo, R P]] | |||
[[Category: Hybrid state]] | [[Category: Hybrid state]] | ||
[[Category: Ire]] | [[Category: Ire]] | ||
Revision as of 13:19, 18 December 2014
Crystal structure of domain III of the Cricket Paralysis Virus IRES RNA
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