3fm8: Difference between revisions
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==Crystal structure of full length centaurin alpha-1 bound with the FHA domain of KIF13B (CAPRI target)== | |||
<StructureSection load='3fm8' size='340' side='right' caption='[[3fm8]], [[Resolution|resolution]] 2.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3fm8]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FM8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FM8 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KIF13B, GAKIN, KIAA0639 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), CENTA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fm8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fm8 RCSB], [http://www.ebi.ac.uk/pdbsum/3fm8 PDBsum]</span></td></tr> | |||
</table> | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fm/3fm8_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Phosphatidylinositol 3,4,5-triphosphate (PIP3) plays a key role in neuronal polarization and axon formation. PIP3-containing vesicles are transported to axon tips by the kinesin KIF13B via an adaptor protein, centaurin alpha1 (CENTA1). KIF13B interacts with CENTA1 through its forkhead-associated (FHA) domain. We solved the crystal structures of CENTA1 in ligand-free, KIF13B-FHA domain-bound, and PIP3 head group (IP4)-bound conformations, and the CENTA1/KIF13B-FHA/IP4 ternary complex. The first pleckstrin homology (PH) domain of CENTA1 specifically binds to PIP3, while the second binds to both PIP3 and phosphatidylinositol 3,4-biphosphate (PI(3,4)P(2)). The FHA domain of KIF13B interacts with the PH1 domain of one CENTA1 molecule and the ArfGAP domain of a second CENTA1 molecule in a threonine phosphorylation-independent fashion. We propose that full-length KIF13B and CENTA1 form heterotetramers that can bind four phosphoinositide molecules in the vesicle and transport it along the microtubule. | |||
Phosphorylation-independent dual-site binding of the FHA domain of KIF13 mediates phosphoinositide transport via centaurin {alpha}1.,Tong Y, Tempel W, Wang H, Yamada K, Shen L, Senisterra GA, Mackenzie F, Chishti AH, Park HW Proc Natl Acad Sci U S A. 2010 Nov 5. PMID:21057110<ref>PMID:21057110</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
== | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Arrowsmith, C H | [[Category: Arrowsmith, C H]] | ||
[[Category: Bochkarev, A | [[Category: Bochkarev, A]] | ||
[[Category: Bountra, C | [[Category: Bountra, C]] | ||
[[Category: Edwards, A M | [[Category: Edwards, A M]] | ||
[[Category: MacKenzie, F | [[Category: MacKenzie, F]] | ||
[[Category: Park, H | [[Category: Park, H]] | ||
[[Category: | [[Category: Structural genomic]] | ||
[[Category: Shen, L | [[Category: Shen, L]] | ||
[[Category: Tempel, W | [[Category: Tempel, W]] | ||
[[Category: Tong, Y | [[Category: Tong, Y]] | ||
[[Category: Weigelt, J | [[Category: Weigelt, J]] | ||
[[Category: Atp-binding]] | [[Category: Atp-binding]] | ||
[[Category: Cytoskeleton]] | [[Category: Cytoskeleton]] | ||
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[[Category: Phosphoprotein]] | [[Category: Phosphoprotein]] | ||
[[Category: Sgc]] | [[Category: Sgc]] | ||
[[Category: Transport protein-hydrolase activator complex]] | [[Category: Transport protein-hydrolase activator complex]] | ||
[[Category: Zinc-finger]] | [[Category: Zinc-finger]] | ||
Revision as of 13:41, 18 December 2014
Crystal structure of full length centaurin alpha-1 bound with the FHA domain of KIF13B (CAPRI target)
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Proteopedia Page Contributors and Editors (what is this?)
Categories:
- Homo sapiens
- Arrowsmith, C H
- Bochkarev, A
- Bountra, C
- Edwards, A M
- MacKenzie, F
- Park, H
- Structural genomic
- Shen, L
- Tempel, W
- Tong, Y
- Weigelt, J
- Atp-binding
- Cytoskeleton
- Gap
- Gtpase activation
- Kinesin
- Metal binding protein
- Metal-binding
- Microtubule
- Motor protein
- Nucleotide-binding
- Nucleus
- Phosphoprotein
- Sgc
- Transport protein-hydrolase activator complex
- Zinc-finger
