1qbk: Difference between revisions
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[[Image:1qbk.gif|left|200px]] | [[Image:1qbk.gif|left|200px]] | ||
'''STRUCTURE OF THE KARYOPHERIN BETA2-RAN GPPNHP NUCLEAR TRANSPORT COMPLEX''' | {{Structure | ||
|PDB= 1qbk |SIZE=350|CAPTION= <scene name='initialview01'>1qbk</scene>, resolution 3.0Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER'>GNP</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''STRUCTURE OF THE KARYOPHERIN BETA2-RAN GPPNHP NUCLEAR TRANSPORT COMPLEX''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1QBK is a [ | 1QBK is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QBK OCA]. | ||
==Reference== | ==Reference== | ||
Structure of the nuclear transport complex karyopherin-beta2-Ran x GppNHp., Chook YM, Blobel G, Nature. 1999 May 20;399(6733):230-7. PMID:[http:// | Structure of the nuclear transport complex karyopherin-beta2-Ran x GppNHp., Chook YM, Blobel G, Nature. 1999 May 20;399(6733):230-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10353245 10353245] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: GNP]] | [[Category: GNP]] | ||
[[Category: MG]] | [[Category: MG]] | ||
[[Category: heat | [[Category: heat repeat]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:35:28 2008'' | ||
Revision as of 11:35, 20 March 2008
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| 1qbk, resolution 3.0Å | |||||||||||||
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| Ligands: | MG and GNP | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
STRUCTURE OF THE KARYOPHERIN BETA2-RAN GPPNHP NUCLEAR TRANSPORT COMPLEX
Overview
Transport factors in the karyopherin-beta (also called importin-beta) family mediate the movement of macromolecules in nuclear-cytoplasmic transport pathways. Karyopherin-beta2 (transportin) binds a cognate import substrate and targets it to the nuclear pore complex. In the nucleus, Ran x GTP binds karyopherin-beta2 and dissociates the substrate. Here we present the 3.0 A structure of the karyopherin-beta2-Ran x GppNHp complex where GppNHp is a non-hydrolysable GTP analogue. Karyopherin-beta2 contains eighteen HEAT repeats arranged into two continuous orthogonal arches. Ran is clamped in the amino-terminal arch and substrate-binding activity is mapped to the carboxy-terminal arch. A large loop in HEAT repeat 7 spans both arches. Interactions of the loop with Ran and the C-terminal arch implicate it in GTPase-mediated dissociation of the import-substrate. Ran x GppNHp in the complex shows extensive structural rearrangement, compared to Ran GDP, in regions contacting karyopherin-beta2. This provides a structural basis for the specificity of the karyopherin-beta family for the GTP-bound state of Ran, as well as a rationale for interactions of the karyopherin-Ran complex with the regulatory proteins ranGAP, ranGEF and ranBP1.
Disease
Known diseases associated with this structure: Deafness, mitochondrial, modifier of OMIM:[610230], Osteolysis, familial expansile OMIM:[603499], Paget disease of bone OMIM:[603499]
About this Structure
1QBK is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of the nuclear transport complex karyopherin-beta2-Ran x GppNHp., Chook YM, Blobel G, Nature. 1999 May 20;399(6733):230-7. PMID:10353245
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