3mq1: Difference between revisions
From Proteopedia
Jump to navigationJump to search
m Protected "3mq1" [edit=sysop:move=sysop] |
No edit summary |
||
| Line 1: | Line 1: | ||
==Crystal Structure of Dust Mite Allergen Der p 5== | |||
<StructureSection load='3mq1' size='340' side='right' caption='[[3mq1]], [[Resolution|resolution]] 2.80Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3mq1]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Dermatophagoides_pteronyssinus Dermatophagoides pteronyssinus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MQ1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MQ1 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DERP5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6956 Dermatophagoides pteronyssinus])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mq1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mq1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3mq1 RCSB], [http://www.ebi.ac.uk/pdbsum/3mq1 PDBsum]</span></td></tr> | |||
</table> | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mq/3mq1_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Group 5 allergens from house dust mites elicit strong IgE antibody binding in mite-allergic patients. The structure of Der p 5 was determined by x-ray crystallography to better understand the IgE epitopes, to investigate the biologic function in mites, and to compare with the conflicting published Blo t 5 structures, designated 2JMH and 2JRK in the Protein Data Bank. Der p 5 is a three-helical bundle similar to Blo t 5, but the interactions of the helices are more similar to 2JMH than 2JRK. The crystallographic asymmetric unit contains three dimers of Der p 5 that are not exactly alike. Solution scattering techniques were used to assess the multimeric state of Der p 5 in vitro and showed that the predominant state was monomeric, similar to Blo t 5, but larger multimeric species are also present. In the crystal, the formation of the Der p 5 dimer creates a large hydrophobic cavity of approximately 3000 A(3) that could be a ligand-binding site. Many allergens are known to bind hydrophobic ligands, which are thought to stimulate the innate immune system and have adjuvant-like effects on IgE-mediated inflammatory responses. | |||
Der p 5 crystal structure provides insight into the group 5 dust mite allergens.,Mueller GA, Gosavi RA, Krahn JM, Edwards LL, Cuneo MJ, Glesner J, Pomes A, Chapman MD, London RE, Pedersen LC J Biol Chem. 2010 Aug 13;285(33):25394-401. Epub 2010 Jun 9. PMID:20534590<ref>PMID:20534590</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Dermatophagoides pteronyssinus]] | [[Category: Dermatophagoides pteronyssinus]] | ||
[[Category: Chapman, M D | [[Category: Chapman, M D]] | ||
[[Category: Cuneo, M J | [[Category: Cuneo, M J]] | ||
[[Category: Edwards, L L | [[Category: Edwards, L L]] | ||
[[Category: Glesner, J | [[Category: Glesner, J]] | ||
[[Category: Gosavi, R A | [[Category: Gosavi, R A]] | ||
[[Category: Krahn, J M | [[Category: Krahn, J M]] | ||
[[Category: London, R E | [[Category: London, R E]] | ||
[[Category: Mueller, G A | [[Category: Mueller, G A]] | ||
[[Category: Pedersen, L C | [[Category: Pedersen, L C]] | ||
[[Category: Pomes, A | [[Category: Pomes, A]] | ||
[[Category: Allergen]] | [[Category: Allergen]] | ||
[[Category: Dust mite]] | [[Category: Dust mite]] | ||
Revision as of 16:03, 18 December 2014
Crystal Structure of Dust Mite Allergen Der p 5
| ||||||||||||
